DNA DAMAGE, PROMOTION AND THE PROOXIDANT STATE

提出了一个研究项目，调查的作用， 在致癌过程中细胞中的促氧化状态。 的 调查将需要识别，隔离和 表征来自DNA寡聚体的病变， 与活性氧的影响有关。 这些 在培养物中暴露的细胞的DNA中也将寻找损伤 到促氧化剂条件。 将在本发明中使用的细胞 研究是“正常”和“起始”系表皮细胞 对晋升的反应不同 条件和试剂 这可能导致活性氧物质的产生，例如 作为自氧化剂（例如抗坏血酸）或佛波醇酯（例如， PMA）将调查其促进 转型 这项研究旨在检验以下假设： 促进剂通过修饰癌细胞的结构而影响癌发生。 基因组

A research project is proposed for investigating the role of prooxidant states in the cell on the carcinogenesis process. The investigation would entail the identification, isolation and characterization of lesions from DNA oligomers that are associated with the effects of reactive oxygen species. These lesions will be sought also in the DNA of cells exposed in culture to prooxidant conditions. The cells to be employed in the investigation are epidermal cells of "normal" and "initiated" lines which respond differently to promotion. Conditions and agents which may lead to the generation of reactive oxygen species, such as autoxidizing agents (e.g. ascorbic acid) or phorbol esters (e.g. PMA) will be investigated for their ability to promote transformation. The study is designed to test the hypothesis that promoting agents affect carcinogenesis by a modification of the genome.