High Resolution Mass Spectrometer to support Proteomic Research across the Southern 4 Proteomic Consortium

蛋白质是细胞的主要组成部分，具有广泛的生物学功能。蛋白质组学是对蛋白质的表达、功能和相互作用的系统研究。蛋白质组学中使用的关键技术是质谱（MS）和液相色谱，并在称为质谱仪的仪器上进行;和高效液相色谱（HPLC）系统。结合使用，这两种仪器设计用于分离和鉴定从细胞、组织和生物流体中获得的蛋白质、蛋白质片段（肽）和修饰蛋白质。它们使我们能够比较不同条件下的蛋白质量（例如，比较正常细胞和癌细胞之间的蛋白质水平），并鉴定在特定生物过程中重要的蛋白质，包括正常蛋白质和在人类疾病中重要的蛋白质。该应用程序将支持购买先进的质谱仪和HPLC系统，该系统具有增强的能力，可以在非常低的水平上测量这些蛋白质和肽的量，并具有高精度。该仪器已被Southern 4 Proteomic Consortium要求，并将成为英格兰南部蛋白质组学中心的一部分，该中心将允许几所大学使用这种最先进的仪器。该联盟还提供了一个论坛，以交流蛋白质组学的专业知识和知识，并培训研究人员在最新的蛋白质组学技术。该仪器位于南安普顿大学蛋白质组研究中心，其容量的增加和先进能力将使人们能够在具有战略重要性的领域内进行广泛的蛋白质组研究，包括生物安全研究委员会许多优先领域内的研究，包括整个生命过程中的健康老龄化;生物能源，为更绿色、可持续的未来产生新的替代燃料;粮食、营养和健康;提高农业生产的可持续性;生物科学的系统方法;合成生物学和数据驱动的生物学。

New instrumentation for increased capability in Proteomic ResearchProteins are a major building block of cells and perform a huge range of biological functions. Proteomics is the systematic study of the expression, functions and interactions of proteins. The key techniques used in proteomics are mass spectrometry (MS) and liquid chromatography and are performed on instruments called Mass Spectrometers; and High Performance Liquid Chromatography (HPLC) systems. In combination, these two instruments are designed to separate and identify proteins, fragments of proteins (peptides) and modified proteins obtained from cells, tissues and biofluids. They allow us to compare the amounts of proteins between different conditions (e.g., comparison of protein levels between normal cells and cancer cells), and to identify proteins important within a specific biological process including normal ones and those important in human diseases. This application will support the purchase of an advanced Mass Spectrometer and HPLC system with an enhanced ability to measure the amounts of these proteins and peptides at very low levels and with high accuracy.The instrumentation has been requested by the Southern 4 Proteomic Consortium and will form part of a Proteomics hub in the South of England that will allow several universities to use this state-of-the-art instrumentation. This consortium also provides a forum to exchange proteomic expertise and knowledge; and to train researchers in the latest proteomic techniques. Housed within the Centre for Proteomic Research at the University of Southampton, the increased capacity and advanced capabilities of this instrumentation will permit a wide range of proteomic studies within areas of strategic importance, including those within many BBSRC priority areas including healthy aging across the lifecourse; bioenergy, generating new replacement fuels for a greener, sustainable future; food, nutrition and health; sustainability enhancing agricultural production; systems approaches to the biosciences; synthetic biology and data-driven biology.