BBSRC Embrapa - Developing mitochondrial mediated resilience to abiotic stress during plant reproduction.

该项目将汇集来自英国和巴西的研究小组，他们正在研究小麦高温和干旱胁迫、花粉发育和线粒体功能的不同方面，以解决生殖高温和干旱胁迫期间发生的分子遗传和生理事件。这将使种质的开发对开花期间的环境胁迫具有更强的适应能力。诺丁汉大学(UON)-扎威尔逊教授：小麦的生殖发育和雄性可育性。在花粉发育的分子调控和基因网络从模式到作物的转化方面的专业知识。这已经确定了保守的转录因子，这些转录因子正被探索为杂交育种中控制作物肥力的工具。小麦开花适应性与开花时间、雄蕊结构和花药开裂的关系研究。温度胁迫对花粉发育的影响，目标是确定在温度胁迫下的恢复力特征。-I King教授和J King博士：祖先导入计划通过转移一系列物种的整个基因组，将遗传变异从其远亲转移到小麦中，例如黑麦、山羊草、Ambylopyrum muticum、Triticum urartu、Thinopyrum bessarabicum、Th。Argatum，Th.Intermediate，Th.Pitticum，T.timopheevii，转入小麦的小重叠染色体片段。虽然还处于初步阶段，但带有该计划中的导入基因的衍生品已经在研究一系列特征，例如对非生物胁迫的耐受性、化学成分、形态特征。-Assoc教授J Foulkes：小麦的资源利用效率：小麦生理学专业知识-专注于耳朵肥力和资源利用效率。包括(I)穗育性的生理和遗传基础；同化物分配到耳朵的阈值和替代汇。与提高分蘖效率、小穗原基数量和小花育性有关的新的穗育性基因(Ii)氮素利用效率的生理和遗传基础以及(Iii)水分利用效率。巴西研究人员-坎皮纳大学擅长线粒体的分子遗传操作。Paulo Arruda-(PI)分子生物学，遗传学，基因组学，Natalia Verza-(博士后)分子生物学，代谢工程Pedro Barreto-(pos-Doc)分子生物学，基因组学，线粒体代谢Juliana Yassitepe-(EmbRapa研究员)GeneticGeraldo cançado(EmbRapa研究员)转基因方法。基础设施：-Bioscions，UON拥有一流的研究设施，包括专门建造的分子实验室，具有最先进的Micro-CT成像设备、受控环境设备的成像设备；研究环境有利于在协作、跨学科的环境中进行尖端研究。ZAW是植物综合生物学中心(CPIB)的一部分，CPIB是一个主要的国际中心，旨在开发植物发育的动态和多尺度模型，涉及到关于动态激素通量、细胞和器官生长、基因表达、蛋白质和代谢物图谱以及组织力学的数据采集。CPIB将为数据分析、建模提供更多支持，并为数据传播提供平台。

This project will bring together groups from the UK and Brazil who are working on different aspects of heat and drought stress, pollen development and mitochondrial function in wheat to address the molecular genetic and physiological events occurring during reproductive heat and drought stress. This will enable development of germplasm with enhanced resilience to environmental stress during flowering.University of Nottingham(UoN)-Prof ZA Wilson: Reproductive development and male fertility in wheat. Expertise in the molecular regulation of pollen development and translation of gene networks from models, to crops. This has identified conserved transcription factors, which are being explored as tools to control crop fertility for hybrid breeding. Investigation of flowering adaptations in wheat associated with flowering time, stamen structure and anther dehiscence. The impact of temperature stress of pollen development, with the target of identifying traits for resilience during temperature stress.-Prof I King & Dr J King: Ancestral Introgression Programme to transfer genetic variation into wheat from its distant relatives, by transferring the entire genome of a range of species, e.g. Secale cereale, Aegilops speltoides, Ambylopyrum muticum, Triticum urartu, Thinopyrum bessarabicum, Th. elongatum, Th. intermedium, Th. ponticum, T. timopheevii, into wheat in small overlapping chromosome segments. While still at a preliminary stage derivatives carrying introgressions from this programme are already under study for a wide range of traits, e.g. tolerance to abiotic stress, chemical composition, morphological traits.-Assoc Prof J Foulkes: Resource-use efficiency in wheat: Expertise in wheat physiology- focusing on ear fertility and resource-use efficiency. Includes (i) physiological and genetic basis of ear fertility; thresholds for assimilate partitioning to the ear and alternative sinks. Novel ear-fertility genes related to increased efficiency of tillering, spikelet primordia number and floret fertility (ii) physiological and genetic basis of N-use efficiency and (iii) water-use efficiency.Brazilian Researchers - Universidade Estadual de CampinasExpertise in Molecular Genetic manipulation of Mitochondria.Paulo Arruda - (PI) Molecular biology, genetics, genomics,Natalia Verza - (post-doc) Molecular biology, metabolic engeneeringPedro Barreto - (pos-doc) Molecular Biology, genomics, mitochondrial metabolismJuliana Yassitepe - (Embrapa researcher) GeneticsGeraldo Cançado - (Embrapa researcher) Transgenic approaches.Infrastructure:-Biosciences, UoN has excellent research facilities, including purpose built molecular labs, imaging facilities with state-of-the art Micro-CT Imaging Facility, Controlled Environment facilities; the research environment is conducive to cutting-edge research, in a collaborative, interdisciplinary environment. ZAW is part of the Centre for Plant Integrative Biology (CPIB), a major international centre to develop dynamic and multiscale models of plant development, involving data capture on dynamic hormone fluxes; cell & organ growth; gene expression, protein & metabolite profiles and tissue mechanics. CPIB will provide added support for data analysis, modeling and provide a platform for data dissemination.