NEUROTRANSMITTERS AND CYTOKINES IN TOOTH MOVEMENT

本次调查的目的是确定是否有因素 由神经元和循环细胞产生的调节反应， 牙周膜（PDL）和牙槽骨细胞的机械应力， 特别是当其在正牙移动期间应用时。 的 具体目标是：1）定位神经肽P物质， 血管活性肠肽，甲硫氨酸脑啡肽，降钙素基因， 相关肽和细胞因子白细胞介素1 α，1 β，白细胞介素 2、干扰素-γ和肿瘤坏死因子-α; 2）评估效果 在体外骨重建测试系统上的这些试剂中的每一种的浓度; 3） 研究这些药物在局部给药后是否与PDL中的靶细胞结合， 体内施用;以及4）确定是否存在特异性抗体， 与受体附着区域共有的表位位点结合， 能够在体外骨重建中抑制这些试剂 系统 计划进行四项实验。 首先，每个代理都将本地化 通过免疫组织化学染色，在猫的颌骨切片中， 将远端平移犬1小时至 28 d. 在第二种方法中，将每种试剂加入小鼠跟骨培养物中， 研究其对体外骨重建或PDL培养的直接作用 成纤维细胞，以确定刺激的成纤维细胞是否产生因子 影响骨骼重塑 在第三种情况下，将注射药剂 直接进入猫PDL体内，并进行测定 每种药物结合的免疫化学和放射自显影 PDL靶细胞。 在第四种情况下，每种药剂的特异性抗体将 将其与每种试剂一起加入小鼠颅盖培养物中， 试图在体外抑制这些药物对骨重建的影响。 该项目旨在增加有关生物化学方面的知识 正畸牙齿移动。 这种信息的作用方式， 神经和炎症细胞的特定产物可能导致 发展新的诊断和治疗手段。

The objective of this investigation is to determine whether factors produced by neuronal and circulatory cells modulate the response be periodontal ligament (PDL) and alveolar bone cells to mechanical stress, particularly as it is applied during orthodontic tooth movement. The specific aims are to: 1) localize the neuropeptides substance P, vasoactive intestinal peptide, methionine-enkephalin, calcitonin gene- related peptide and the cytokines Interleukin 1 alpha, 1 beta, Interleukin 2, interferon-gamma and tumor necrosis factor-alpha; 2) assess the effects of each of these agents on an in vitro bone remodeling test system; 3) investigate whether these agents bind to target cells in the PDL upon local administration in vivo; and 4) determine whether specific antibodies, which bind to epitope sites that are common to the region of receptor attachment, are capable of inhibiting these agents in an in vitro bone remodeling system. Four experiments are planned. In the first, each agent will be localized by immunohistochemical staining in jaw sections of cats whose one maxillary canine would be translated distally for period of time ranging from 1h to 28d. In the second, each agent will be added to mouse calcaria cultures to study its direct effect on bone remodeling in vitro; or to cultures of PDL fibroblasts to determine whether stimulated fibroblasts produce factors which affect bone remodeling. In the third, agents will be injected directly into cat PDL in vivo and determinations will be made immunohistochemically and autoradiographically of the binding of each agent to PDL target cells. In the fourth, specific antibodies to each agent will be added to mouse calvaria cultures, together with each agent, in an attempt to inhibit the effects of these agents on bone remodeling in vitro. This project is aimed at increasing knowledge about the biochemical aspects of orthodontic tooth movement. Such information on the mode of action of specific products of nervous and inflammatory cells may lead to the development of new diagnostic and therapeutic means in orthodontics.