EXTRINSIC CONTROL OF GASTROINTESTINAL MOTILITY

长期目标是了解椎前神经节 调节/调节结肠、小肠和胃运动。 朝向 为此，规划了四个主要研究领域。 在第一个， 实验旨在确定P物质的功能作用， 血管活性肠多肽，脑啡肽和铃蟾肽在 调节结肠运动。 第二，在第一个问题上， 突触输入和肌壁内的电生理特性 神经节细胞将被确定，认为这些旁束 神经节将中枢指令分配给肌间神经丛， 在影响或控制胃运动中起作用。 第三 区域，胃和肠运动的交感神经调节将是 通过确定节前神经元之间的精确排列 来自胸腰段脊髓和神经节细胞的纤维 腹腔神经节 在最后一个领域，实验的目的是 通过确定腹部椎前神经节的连线， 神经节内和神经节间连接的性质和程度 神经节细胞在相同和相邻的神经节，并通过确定 椎前神经节细胞的局部（空间）排列 神经节支配胃和胃的不同功能区域 小肠 方法将涉及免疫学 包括放射免疫测定和免疫药理学方法的技术， 测量胃肠道段中的管腔内压力 束，来自单个神经节细胞的细胞内记录， 辣根过氧化物酶标记形态学技术 神经元 计划进行体外和体内实验。 豚鼠 猫将用于椎旁神经节的研究， 负鼠将被用于研究的壁内神经节细胞的 胃 这些研究可能特别适用于 胃肠运动包括肠梗阻或粘连肠， 假性梗阻、胃张力减退和自主神经功能障碍 胃和肠运动。 这些研究也可能揭示一些关于 内脏神经元与神经元之间联系可能的神经肽能基础 疼痛和胃肠运动异常。

The long term objective is to understand how the prevertebral ganglia regulate/modulate colonic, small intestinal and gastric motility. Toward this objective four major areas of study are planned. In the first, experiments are designed to determine the functional role of substance P, vasoactive intestinal polypeptide, the enkephalin and bombesin in the regulation of colonic motility. In the second, the pattern and nature of synaptic input to and the electrophysiological properties of intramural ganglion cells will be determined with the view that these parafascicular ganglion distribute central commands to the myenteric plexus and thereby play a role in influencing or controlling gastric motility. In the third area, sympathetic regulation of gastric and intestinal motility will be addressed by identifying the precise arrangement between preganglionic fibers arising from the thoraco-lumbar spinal cord and ganglion cells in the celiac ganglia. In the final area, experiments are designed to delineate the wiring of the abdominal prevertebral ganglia by determining the nature and extent of intra- and inter-ganglionic connections between ganglion cells in the same and adjacent ganglia and by determining the topographical (spatial) arrangement of ganglion cells in the prevertebral ganglia which innervate the stomach and different functional regions of the small intestine. The methods of approach will involve immunological techniques including radioimmunoassay and immunopharmacological methods, measurement of intraluminal pressure in segments of the gastrointestinal tract, intracellular recordings from individual ganglion cells and morphological techniques using the horseradish peroxidase method of marking neurons. Both in vitro and in vivo experiments are planned. Guinea pigs and cats will be used for studies on the pevertebral ganglia whereas opossum will be used for studies on intramural ganglion cells of the stomach. These studies may be particularly relevant to disturbances in gastrointestinal motility including ileus or adynamic bowel, pseudo-obstruction, gastric atonia and autonomic dysfunction affecting gastric and intestinal motility. These studies may also shed some light on the possible neural peptidergic basis for an association between visceral pain and gastrointestinal motor abnormalities.