MASS SPECTROMETRY OF INVOLATILE BIOMOLECULES

质谱法是分析生物样品的一种不可或缺的技术。 重要的生物化合物。 因此，开发用于 大的、不稳定的、极性分子的质谱分析是非常重要的。 生物医学、生物化学和生物技术的发展 以理工科为重 我们的总体目标是扩大有机食品的效用， 生物医学和生物化学中的质谱。 我们的具体目标如下：1.实验研究 控制二次离子喷射的物理和化学过程 在用keV粒子轰击期间来自固体和液体基质; 2. 制定和测试沉积和分配的机制假设 能量进入有机样品基质，并用于碎裂和电离 溅射分子; 3.利用基本过程的知识 优化二次离子源设计和实验方案， 高分子量、不稳定、极性分子的质谱分析 生物化学和生物学的兴趣。 液态金属离子源 是实现我们实验目标的关键 我们率先使用 在二次离子质谱中， （SIMS）。 当重要的生物分子 由于质量范围、灵敏度或 复合类型。 最近，SIMS采用原子离子或 中性原子初级束和液体样品基质 显著地消除了对质谱的许多限制， 分析热不稳定的高分子量有机化合物。 它 我们的前提是， 分子二次离子质谱将对应用质量 光谱学在生物医学和生物化学中的重要问题。 这个， 那么，我们的具体目标是如何与我们长期搁浅的将军联系在一起的， objective.

Mass spectrometry is an indispensable technique for the analysis of biologically important compounds. Thus, the development of techniques for mass spectrometric analysis of large, labile, polar molecules is of great importance to progress in the biomedical, biochemical, and biological sciences. It is our general objective to extend the utility of organic mass spectrometry in biomedicine and biochemistry. Our specific aims are as follow: 1. experimentally investigate the physical and chemical processes which govern the ejection of secondary ions from solid and liquid matrices during bombardment with keV particles; 2. formulate and test mechanistic hypotheses for deposition and partitioning of energy into organic sample matrices and for fragmentation and ionization of sputtered molecules; 3. utilize knowledge of the fundamental processes to optimize source designs and experimental protocols for secondary ion mass spectrometry of high molecular weight, labile, polar molecules of biochemical and biological interest. The liquid metal ion primary source is the key to achieving our experimental aims. We have pioneered the use of this unique and versatile ion source in secondary ion mass spectrometry (SIMS). The usefulness of mass spectrometry is reduced when important biomolecules cannot be analyzed due to limitations on mass range, sensitivity or compound types. Relatively recently, SIMS employing either atomic ion or neutral atom primary beams and liquid sample matrices has contributed significantly to removing numerous restrictions on the mass spectrometric analysis of thermally labile, high molecular weight, organic compounds. It is our premise that research which leads to enhancement of the capabilities of molecular SIMS will have made a major contribution toward applying mass spectrometry to important problems in biomedicine and biochemistry. This, then, is how our specific aims are related to our long-stranding general objective.