Ultra High-Throughput Sequencing for Norwich Research Park and the UK National Capability in Genomics

快速而廉价地对活体基因组进行测序的能力使生物和生物医学研究发生了革命性的变化。在过去的10年里，使这类研究成为可能的技术有了巨大的进步，我们理解数据的能力也得到了极大的提高。基因组测序已经从需要定制的工厂规模设备的活动变成了需要单一仪器的事情，可以在英国各地的大学实验室运行，现在甚至可以在手持设备中运行。然而，随着这一过程变得更快、更便宜，我们现在可以设计更大、更强大的研究，仍然需要定制的设施来处理现在可能进行的更大规模的研究设计。在这里，我们建议购买最新迭代的短读测序技术NovaSeq 6000，并将其安装在厄勒姆研究所，该研究所拥有BBSRC国家基因组和单细胞分析能力。这一仪器将比EI以前可能实现的测序速度更快、成本更低。EI是该仪器的理想环境，因为它可以实现上游样品准备的自动化。它拥有一支训练有素的员工队伍，拥有完善的培训计划和服务交付人员的职业发展。EI还拥有非常好的IT基础设施和一支支持测序实验室的生物信息学家团队。EI计划在六个月内安装该平台，对其进行基准测试，并使其可供英国生物科学界使用。

The ability to rapidly and cheaply sequence the genomes of living organisms has revolutionised biological and biomedical research. In the last 10 years the technology to enable this type of research as improved massively, as has our ability to understand the data. Genome sequencing has changed from an activity that required a bespoke factory scale facility to something that requires a single instrument which can be run in university labs across the UK and now even in hand-held devices. However, as the process has become quicker and cheaper, we can now design much larger and more powerful studies and there is still a need for bespoke facilities that can handle the larger study designs that are now possible. Here we propose to purchase the most recent iteration of short-read sequencing technology, The NovaSeq 6000 and install it at the Earlham Institute, which hosts the BBSRC National Capability in Genomics and Single Cell Analysis. This instrument will enable much more rapid and much cheaper sequencing than was previously possible at EI. The EI is an ideal environment for this instrument as it has access to automation for upstream sample prep. It has a highly trained workforce with a well-established training program and career progression for service delivery staff. EI also has very good IT infrastructure and a team of Bioinformaticians supporting the sequencing lab. Over a six month period the EI plan to install the platform, benchmark it and make it accessible to the UK bioscience community.