LUNG CLEARANCE DURING REPEATED EXPOSURES TO H2SO4 & O3

本项目研究间歇性（2/hd，5/d wk） O3和H2SO 4暴露对清除防御机制的影响 以家兔为动物模型， 气管支气管粘膜纤毛清除率、肺泡清除率和 通过灌洗获得的巨噬细胞的功能特性 将对暴露的动物进行评估。 此外， 将检查支气管粘膜和肺泡上皮， 暴露期间选定时间的肺组织组织学 系列. 暴露将持续长达12个月，一群兔子 分别于4、8、12和15个月处死。 后者规定， 恢复期，以检查影响的解决方案。 间隙 使用放射性标记示踪剂在未镇静动物中进行研究 气溶胶 该项目的结果将有助于阐明 清除功能的改变可能在 慢性肺疾病的发病机制，和时间 一些因素的变化之间的关系， 影响防御功能。

This project examines the effects of intermittent (2/hd, 5/d wk) exposures to O3 and H2SO4 upon clearance defense mechanisms of the lung, using the rabbit as the animal model. Tracheobronchial mucociliary clearance, alveolar clearance and functional properties of macrophages obtained by lavage from exposed animals will be assessed. In addition, permeability of bronchila mucosa and alveolar epithelium will be examined, as will histology of lung tissue at selected times during the exposure series. Exposures will last up to 12 mo., with groups of rabbits sacrificed at 4, 8, 12 and 15 mo. The latter provides for a recovery period to examine resolution of effects. Clearance is studied in unsedated animals using radioactively tagged tracer aerosols. The results of this project will help in elucidation of the role that alteration in clearance function may have in pathogenesis of chronic lung disease, and the temporal relationship between changes in a number of factors which could affect defense functions.