MOLECULAR APPROACHES TO CELL ULTRASTRUCTURE

我们将开展以下几个项目：1）高分辨率免疫电子 一些最近发现的连接的显微定位 在各种膜-微丝附着位点的蛋白质; 2） 继续寻找在这些网站的完整膜蛋白，使用 单克隆抗体结合免疫细胞化学筛选法 定位; 3）继续研究免疫定位， 完整细胞内蛋白质的磷酸酪氨酸残基，使用特异性 产生针对磷酸酪氨酸的单克隆抗体; 4）我们的扩展 免疫电镜研究的细胞内途径的血清 培养肝细胞的蛋白分泌; 5）探索 我们已经证明了这种关联的分子基础， 高尔基体与细胞中的微管组织中心。

We will carry out several projects: 1) the high resolution immunoelectron microscopic localization of a number of recently discovered junctional proteins at a variety of membrane - microfilament attachment sites; 2) continued search for integral membrane proteins at these sites, using a monoclonal antibody approach combined with selection by immunocytochemical localization; 3) continued studies of the immunolocalization of phosphotyrosine residues of proteins inside intact cells, using specific monoclonal antibodies generated to phosphotyrosine; 4) the extension of our immunoelectron microscopic studies of the intracellular pathways of serum protein secretion in cultured hepatocytes; and 5) the exploration of the molecular basis for the association which we have demonstrated between the Golgi apparatus and the microtubule organizing center in cells.