MOLECULAR APPROACHES TO CELL ULTRASTRUCTURE

我们将开展以下几个项目：1)高分辨率免疫电子 若干新近发现的结合部的显微定位 各种膜-微丝附着部位的蛋白质；2) 继续在这些位点寻找完整的膜蛋白，使用 结合免疫细胞化学筛选的单抗方法 定位；3)免疫定位的继续研究 完整细胞内蛋白质的磷酸酪氨酸残留量，使用特定的 产生的抗磷酸化酪氨酸的单抗；4)我们的 血清细胞内途径的免疫电子显微镜研究 培养肝细胞的蛋白质分泌；5)肝细胞蛋白质分泌的研究。 我们已经证明的相互作用的分子基础 高尔基体和细胞内的微管组织中心。

We will carry out several projects: 1) the high resolution immunoelectron microscopic localization of a number of recently discovered junctional proteins at a variety of membrane - microfilament attachment sites; 2) continued search for integral membrane proteins at these sites, using a monoclonal antibody approach combined with selection by immunocytochemical localization; 3) continued studies of the immunolocalization of phosphotyrosine residues of proteins inside intact cells, using specific monoclonal antibodies generated to phosphotyrosine; 4) the extension of our immunoelectron microscopic studies of the intracellular pathways of serum protein secretion in cultured hepatocytes; and 5) the exploration of the molecular basis for the association which we have demonstrated between the Golgi apparatus and the microtubule organizing center in cells.