PROTEIN LIPID INTERACTION

这项建议的目的是通过遗传学手段继续我们的研究 几个定义明确的系统，其中蛋白质和脂质 互动. 这种互动对大会至关重要， 生物膜的功能。 这些系统是：i） 细菌酶环丙烷脂肪酸合酶，ii） 含脂质噬菌体PR 4的形态发生，和iii） 苹果酸氧化酶，一种被磷脂激活的酶。 环丙烷脂肪酸合成酶是唯一的离散酶 已知其特异性地与 磷脂双层 我们建议准备大量的 该酶的重组DNA技术，并研究其 与其磷脂底物的详细相互作用。 噬菌体PR4 从细菌膜上获得衣壳脂质， 过程是一个很好的模型来研究膜形态发生， 基因手段 我们建议结合遗传学和 生物化学来研究噬菌体脂质 是后天获得的。 苹果酸氧化酶，一个新的项目，是一种酶， 被磷脂激活 我们希望分离出 这种酶在脂质激活中有缺陷， 脂质结合在苹果酸氧化酶的功能。

The aim of this proposal is to continue our study by genetic means of several well-defined systems in which proteins and lipids interact. Such interactions are essential to the assembly and function of biological membranes. The systems are i) the bacterial enzyme cyclopropane fatty acid synthase, ii) morphogenesis of the lipid-containing bacteriophage PR4, and iii) malate oxidase, an enzyme which is activated by phospholipids. Cyclopropane fatty acid synthase is the only discrete enzyme known to specifically react with the nonpolar portion of a phospholipid bilayer. We propose to prepare large quantities of this enzyme by recombinant DNA techniques and to study its detailed interaction with its phospholipid substrate. Phage PR4 acquires its capsid lipid from the bacterial membrane and this process is a good model to study membrane morphogenesis by genetic means. We propose to use a combination of genetics and biochemistry to study the mechanisms whereby the phage lipids are acquired. Malate oxidase, a new project, is an enzyme that is greatly activated by phospholipids. We wish to isolate mutants of this enzyme defective in activation by lipids to determine the role of lipid binding in malate oxidase function.