Understanding how RNA is tethered to chromatin

该项目解决了RNA在细胞中的定位问题。位置是非常重要的，因为任何房地产经纪人会告诉你。哺乳动物细胞产生具有不同功能的广泛的RNA分子。一些众所周知的RNA，如信使RNA，在细胞核中产生，然后输出到细胞质，在那里它们编码蛋白质。许多其他类型的RNA主要存在于细胞核中，其中一些与DNA相连，在那里它们调节RNA的产生并在细胞核的整体结构组织中发挥作用。我们已经发现，HNRNPU蛋白在细胞核中将RNA连接到DNA中起着广泛的作用，并且还可能参与将不同的RNA相互连接。在这个项目中，我们将研究由HNRNPU控制的核RNA：DNA和RNA：RNA拴系机制，并确定这些过程对哺乳动物基因表达的影响。

This project addresses where RNA is localised in the cell. Location is very important as any estate agent will tell you. The mammalian cell produces a wide range of RNA molecules with diverse functions. Some well known RNAs, such as messenger RNA, are produced in the nucleus and then exported to the cytoplasm where they encode for proteins. Many other types of RNA largely reside in the nucleus and some of them are tethered to the DNA where they regulate the production of RNA and play a role in the overall structural organisation of the nucleus. We have discovered that the HNRNPU protein plays a widespread role in tethering RNAs to DNA in the nucleus and additionally is probably involved in tethering different RNAs to each other. In this project we will characterise the nuclear RNA:DNA and RNA:RNA tethering mechanisms controlled by HNRNPU and determine the impact these processes have on mammalian gene expression.