High throughput gentle imaging of cells, embryos and organoids with an inverted lattice light sheet microscope.

剑桥高级成像中心(CAIC)是剑桥大学的生物成像研究机构。它为该地区、国内和国际的研究人员提供了接触最先进的显微镜的机会。CAIC通过充当物理学家、工程师、计算机科学家、化学家和生物学家合作的中心，提高了显微镜产生的价值。自2013年开业以来，CAIC一直与技术进步保持同步，直接开发显微镜，并改善部门、研究所和行业之间获得设备的机会。为了保持卓越，CAIC不断识别和实施满足该地区成像行业迫切需求的尖端成像方法。在这个项目中，我们将在CAIC中安装一种新型的倒格光片显微镜。该设备将填补我们国家实时成像产品组合中的一个关键空白，建立一种在细胞和胚胎实时成像方面提供世界领先水平的能力。与金标共焦显微镜相比，光片显微镜的特点是显著减少了光对被观察标本造成的损害。这一好处被降低的分辨率和限制使用的繁琐的样品安装方案所抵消。在CAIC，我们敏锐地意识到这些局限性，开发了两种定制的立式和卧式光片显微镜。这些阻碍广泛应用的障碍已经被一种新型的显微镜--倒置晶格光片显微镜消除了。这项新技术具有创新的透镜系统，使光片显微镜与所有常见的显微镜载玻片和碟子兼容，其影响是巨大的。在荧光显微镜中，弱光照明的重要性怎么强调都不为过--特别是对于需要对有机物和胚胎进行长期成像的研究项目。研究人员最终将能够不受限制地利用光片显微镜的温和成像，使用多孔板、栅格和幻灯片来成像比以前多一个数量级的标本。实现有机物和胚胎的低光毒性成像将极大地提高成像成功率，减少所需的动物总数，使该显微镜成为使用动物进行研究实现3R的必要工具。在长期成像实验中轻松地对许多样本进行成像的能力消除了选择偏见，并将样本数量增加了十倍，从而极大地改进了数据驱动的生物学。倒置点阵光片显微镜将补充我们现有的实时成像设备组合，从而形成具有世界领先能力的独特实时生物成像设备。

The Cambridge Advanced Imaging Centre (CAIC) is a bioimaging research facility at the University of Cambridge. It provides researchers across the region, nationally and internationally with access to state-of-the-art microscopes. CAIC enhances the value generated by microscopes by acting as a hub for physicists, engineers, computer scientists, chemists and biologists to work together. Since opening in 2013, CAIC has kept pace with technology advances by directly developing microscopes and improving access to equipment among departments, institutes and industry. In order to maintain excellence, CAIC continually identifies and implements cutting-edge imaging methods that meet the pressing needs of the region's imaging community.In this project we will install a novel inverted lattice light sheet microscope into CAIC. This equipment will fill a critical gap in our national live imaging portfolio, establishing a capability that will deliver world leading excellence in live imaging of cells and embryos. The hallmark of light sheet microscopy is a significant reduction in damage caused by light to the specimen under observation when compared to gold-standard confocal microscopes. This benefit is offset by reduced resolution and cumbersome sample mounting protocols which limit use. At CAIC we are keenly aware of these limitations, having developed two customised upright and horizontal light sheet microscopes. These barriers to widespread adoption have been eliminated by a novel microscope - the inverted lattice light sheet microscope. This new technology has an innovative lens system, making light sheet microscopes compatible with all common microscope slides and dishes, the impact of which is enormous.It is difficult to over-emphasise the importance of low-light illumination in fluorescence microscopy - particularly for research projects that require long-term imaging of organoids and embryos. Researchers will finally be able to exploit the gentle imaging of light sheet microscopy in an unrestricted way, using multi-well plates, grids and slides to image an order of magnitude more specimens than before. Enabling low phototoxicity imaging of organoids and embryos will drastically improve success rates in imaging, reducing the total number of animals required, making this microscope an essential tool in achieving the 3Rs in research using animals. The ability to easily image many samples in long-term imaging experiments removes selection bias and increases sample numbers by a factor of ten, leading to greatly improved data driven biology. The inverted lattice light sheet microscope will complement our existing live imaging equipment portfolio, resulting in a unique live bioimaging facility with world-leading capabilities.