STUDY OF PHOTOAFFINITY LABELS BY LASER SPECTROSCOPY

生物大分子的光亲和标记被广泛应用于 确定复杂单位内的特定受体位置。这项技术 与更传统的贴标程序相比，提供了许多优势。 其中之一是潜在反应性的光化学激活可以 生产的标签剂比传统的标签剂更具活性 基态化学试剂。我们将使用纳秒和皮秒 激光光谱学探测产生和随后的细节 引起共价反应的活性分子物种的转化 将标签附着在生物大分子上。我们将尝试 定义可光激活基团的结构特征，以确保 特定的、不可逆的所需受体的标记。通过研究 光生物种与特定官能团的反应速率 通常在生物受体部位发现的群，我们将判断 试剂的实用性和可靠性。我们的调查将集中在 芳香族叠氮化合物和重氮化合物的基础研究 标签中使用的最常见的光化学物质。

Photoaffinity labeling of biological macromolecules is widely used to identify specific receptor sites within complex units. This technique provides numerous advantages over more conventional labeling procedures. One of these is that photochemical activation of latent reactivity can generate labeling agents that are far more reactive than conventional ground-state chemical reagents. We will use nanosecond and picosecond laser spectroscopy to probe the details of the generation and subsequent transformations of the reactive molecular species responsible for covalent attatchment of the label to the biological macromolecule. We will attempt to define the structural features of the photoactivatable group that insure specific, irreversible labeling of the desired receptor. By studying the rates of reaction of the photogenerated species with specific functional groups typically found at biological receptor sites we will judge the utility and reliability of the reagent. Our investigation will focus on fundamental studies of aromatic azides, and of diazo compounds as these are the most common photochemicals used in labeling.