MECHANISMS OF PHAGE EXCLUSION SYSTEM

这项研究的主要目的是了解 T7及其相关基因F排斥的分子机制 噬菌体。这是一个模型系统或宿主与寄生虫的相互作用 其中涉及的基因可以很容易地通过基因方法进行操作。 在这个特殊的排斥系统中积累的知识正在被应用于 其他人则用来确定模型的一般性。 经典和分子遗传学技术被用来 调查与以下相关的多效性生理功能障碍 F-排除。这些包括基因表达的干扰、抑制 DNA复制和膜缺陷。它将被确定是否 阻碍噬菌体发育的主要因素是转录介导的 噬菌体DNA进入细胞。噬菌体蛋白是如何 排除系统的靶点与大肠杆菌DNA旋转酶相互作用 正常的噬菌体发育有待研究。 描述了在这些宿主中改变的突变体的遗传选择 排除系统中涉及的产品。这些变种人应该 帮助确定噬菌体和质粒编码基因的相互作用部位 并提供有关导致 在噬菌体发育过程中的滞留。

The primary objective of this research is to understand the molecular mechanism underlying F-exclusion of T7 and related bacteriophages. This is a model system or host-parasite interactions where the genes involved can be easily manipulated by genetic approaches. Knowledge accrued in this particular exclusion system is being applied to others to determine the generality of the model. Classical and molecular genetic techniques are employed to investigate the pleiotropic physiological dysfunctions associated with F-exclusion. These include perturbations in gene expression, inhibition of DNA replication, and membrane defects. It will be determined if the primary block to phage development is the transcriptionally-mediated entry of phage DNA into the cell. How the phage proteins that are the targets of the exclusion system interact with E. coli DNA gyrase in normal phage development is to be investigated. A genetic selection is described for mutants altered in those host products that are involved in the exclusion system. These mutants should help define the site of interaction of the phage- and plasmid-coded genes and provide information on the primary cellular event that causes the arrest in phage development.