ANESTHESIA-ANALGESIA OF SPINAL NEURONS

这项研究计划的长期目标仍然是： 发展一种简明的生理学描述， 感觉神经元药理学，尤其是与 疼痛，在生理脊髓的背角 完整的清醒的无药物的被束缚的猫 这份提案是独一无二的 因为它将涉及来自单个脊髓的细胞外记录， 完整的清醒动物的神经元。 这将允许进行评估 系统的正常功能，而不受干扰 麻醉、去大脑或脊髓的影响 横切 WDR（multireceptive）神经元，因为它们的 对有害和无害刺激的独特反应， 在许多脊髓损伤的理论中， 传递疼痛信息。 此外，WDR神经元具有 被确定为麻醉剂和镇痛剂的作用部位 毒品 尽管他们在疼痛文学中扮演着重要角色， 然而，WDR神经元的生理意义仍然是开放的 去质疑 最近的结果获得的工作资助， 格兰特揭示了两个关于WDR神经元的重要发现， 完整的动物。 首先，遇到的WDR神经元的数量 在完整动物的脊髓背角中， 从急性研究获得的结果预测。 第二、 巴比妥类麻醉似乎揭示了WDR反应特征 一些脊髓背角神经元。 因为潜在的 WDR神经元在我们理解神经系统中的重要作用 麻醉和镇痛的神经生理学和药理学， 我们必须清楚地认识到 调节宽动态范围神经元响应特性， 生理上完整的清醒的无药物的动物。 因此，这 该提案的具体目标如下：1）确定 如果WDR响应曲线的解蔽是巴比妥类药物特异性的， 效果或是否可以由其他全身麻醉药产生; 2） 确定与过渡相关联的唤醒变化是否 从清醒状态到睡眠状态产生WDR的去掩蔽 反应曲线; 3）确定是否从 蓝斑对掩盖 完整动物中的WDR响应曲线; 4）确定，在 水平的脊髓，什么影响药理阻断 去甲肾上腺素能系统在暴露WDR反应时的作用 数据区.

The long-term objectives of this research plan continue to be the development of a concise description of the physiology and pharmacology of sensory neurons, especially those associated with pain, in the dorsal horn of the spinal cord of physiologically intact, awake, drug free, restrained cats. This proposal is unique in that it will involve extracellular recordings from single spinal neurons in intact, awake, animals. This will permit an evaluation of the systems as they normally function without confounding influences of anesthesia, decerebration, or spinal cord transection. WDR (multireceptive) neurons, because of their unique response to both noxious and non-noxious stimuli, have been assigned a central role in many theories of spinal transmission of pain information. In addition, WDR neurons have been identified as sites of action of anesthetic and analgesic drugs. In spite of their prominent role in the pain literature, however, the physiologic significance of WDR neurons is still open to question. Recent results obtained from work funded by this grant have revealed two important findings about WDR neurons in the intact animal. First, the number of WDR neurons encountered in the spinal dorsal horn of intact animals is much less than would be predicted from results obtained form acute studies. Secondly, barbiturate anesthesia appears to unmask WDR response profiles of some spinal dorsal horn neurons. Because of the potentially important role of WDR neurons in our understanding of the neurophysiology and pharmacology of anesthesia and analgesia, it is essential that we have a clear appreciation of the factors that regulate wide dynamic range neuron response properties in physiologically intact, awake, drug free animals. As such, this proposal has, as its specific aims, the following: 1) to determine if unmasking of WDR response profiles is a barbiturate-specific effect or if it can be produced by other general anesthetics; 2) to determine if changes in arousal associated with the transition form the awake to the sleep state produce unmasking of WDR response profiles; 3) to determine if descending inhibition from the locus coeruleus contributes significantly to the masking of WDR response profiles in intact animals; 4) to determine, at the level of the spinal cord, what effect pharmacological blockage of noradrenergic systems has upon the unmasking of WDR response profiles.