STRUCTURE AND REGULATION OF AN ECDYSONE-RESPONSIVE GENE

果蝇EIP 29/29基因受类固醇激素的调控 激素蜕皮激素 该基因特别令人感兴趣，因为在两个 果蝇细胞系EIP 28/29转录物的丰度增加， 蜕皮激素的早期和可能的主要作用。 这个的结构 可诱导的Kc主要转录单位已被解决。 有证据 在许多果蝇中， 组织中 也有证据表明，替代，重叠转录 这些单位是组织特异性的，其中一些可能是蜕皮激素调节的。 我们提出两条工作路线。 首先，我们建议研究Kc大调 启动子已经确定，目的是确定这些序列 其启动子活性和调节所需的元素。 二是 建议分析EIP 28/29基因在发育中的表达， 特别强调确定各种组织结构的问题， EIP 28/29转录本，描述其调节和表征 他们的蛋白质产品。 拟议研究的结果将有助于我们了解 类固醇激素作用的分子水平，并将丰富我们的 了解这些激素在发育中的作用。

The EIP 29/29 gene of Drosophila melanogaster is regulated by the steroid hormone ecdysone. The gene is of particular interest because in two Drosophila cell lines EIP 28/29 transcripts increase in abundance as a very early and probably primary effect of ecdysone. The structure of this inducible, Kc major transcription unit has been solved. There is evidence that this transcription unit is hormonally induced in many Drosophila tissues. There is also evidence for alternative, overlapping transcription units which are tissue-specific and some of which may be ecdysone-regulated. We propose two lines of work. First, we propose to study the Kc major promotor already identified with the goal of identifying those sequence elements required for its promotor activity and regulation. Second, we propose to analyze the expression of the EIP 28/29 gene in development with special emphasis on the problems of defining the structures of the various EIP 28/29 transcripts, describing their regulation, and characterizing their protein products. The results of the proposed study will contribute to our understanding at the molecular level of steroid hormone action and will enrich our understanding of the roles of such hormones in development.