BRAIN MONOAMINES AND LUTEINIZING HORMONE SECRETION

拟议研究的总体目标是调查 雌二醇和孕酮控制的神经化学机制 黄体生成素和催乳素的分泌 脑下垂体。这项研究将检验卵巢的假说 激素通过激活内源性阿片调节黄体生成素和催乳素的分泌 对药物的释放施加局部控制的多肽系统 去甲肾上腺素、肾上腺素和5羟色胺。它还假设 对黄体生成素和催乳素的单胺能控制被存在或改变 在围产期关键期缺乏睾丸素。这些 假设将通过以下方法进行检验。第一，卵巢 荷尔蒙将以特定的方式给予，以降低或升高 黄体生成素和催乳素，以及这些处理对黄体生成素周转的影响。 我们将评估离散脑核中的5-羟色胺。这将确定 卵巢激素改变5-羟色胺能活动的部位。第二 阶段将尝试建立以下变化之间的因果关系 卵巢5-羟色胺活性与促黄体生成素和催乳素的变化 荷尔蒙。这将涉及到试图改变预期的卵巢 荷尔蒙反馈效应通过产生局部去神经作用 神经毒性吲哚胺，5，7-二羟色胺。第三阶段将测试 卵巢激素是否与内源性阿片神经肽相互作用 影响单胺类物质的释放，调节黄体生成素和催乳素的分泌。这 将通过测试卵巢激素对两者的影响来实现 脑下垂体激素的分泌和单胺的代谢会受到 阿片类激动剂和拮抗剂。该项目的第四个方面将 调查是否对新生儿男性进行阉割或给药 睾丸激素对新生儿女性的影响--改变卵巢激素的操作 成年后控制促黄体生成素和催乳素，也会改变卵巢的作用 激素对促黄体生成素释放激素和单胺代谢的影响。

The overall objective of the proposed research is to investigate the neurochemical mechanisms by which estradiol and progesterone control the secretion of luteinizing hormone (LH) and prolactin (PRL) from the anterior pituitary gland. This research will test the hypothesis that ovarian hormones regulate LH and PRL secretion by activating endogenous opiate peptide systems that exert localized control over the release of norepinephrine, epinephrine and serotonin. It is also hypothesized that monoaminergic control over LH and PRL is modified by the presence or absence of testosterone during a perinatal critical period. These hypotheses will be tested by the following approaches. First, ovarian hormones will be administered in specific regimens that depress or elevate LH and PRL, and the effects of these treatments on the turnover of serotonin in discrete brain nuclei will be assessed. This will identify those sites where ovarian hormones alter serotonergic activity. The second phase will attempt to establish causal relationships between the changes of serotonin activity and the changes in LH and PRL induced by the ovarian hormones. This will involve attempts to alter the expected ovarian hormonal feedback effects by producing local denervations with the neurotoxic indoleamine, 5,7-dihydroxytryptamine. The third phase will test whether ovarian hormones interact with endogenous opiate neuropeptides that influence the release of monoamines to regulate LH and PRL secretion. This will be accomplished by testing whether ovarian hormonal effects on both pituitary hormone secretion and on monoamine turnover can be affected by opiate agonists and antagonists. A fourth aspect of the project will investigate whether castration of neonatal males or administration of testosterone to neonatal females, manipulations that alter ovarian hormonal control over LH and PRL in adulthood, also alter the effects of ovarian hormones on LH-releasing hormone and on monoamine turnover.