BIOSYNTHESIS OF BOVINE LUTEINIZING HORMONE

促性腺激素(黄体生成素、卵泡刺激素和人绒毛膜促性腺激素)由脑垂体和胎盘产生，是关键。 荷尔蒙与人类生殖有关。这项建议涉及 BLH的结构、功能和生物合成是我们的 正在进行的研究。BLH中的碳水化合物结构是独特的，因为它具有 54-0取代N-乙酰半乳糖胺的非还原末端 天冬酰胺连接的碳水化合物单位。取代基的同一性为 没有得到证实。本提案的具体目标是：(1) N-乙酰氨基半乳糖取代基的鉴定。(2) 含有取代基和取代基的酶的特性 N-乙酰氨基半乳糖。(3)聚四氟乙烯的组装机理研究 寡糖-脂类参与BLH碳水化合物的生物合成 主要中间体的分离。(4)黄酒加工工艺研究 低聚糖通过分离转移到蛋白质后 各种中间体和外周糖类的添加。(5)尝试 脱糖化BLH-α和BLH体外全合成的研究 低聚糖脂中低聚糖的转移 中间体，然后是低聚糖的完整加工。 (6)类固醇、促黄体生成素释放因子和环磷酸腺苷对血管紧张素转换酶活性的影响 促黄体生成素的合成和分泌。(7)碳水化合物在体内的作用研究 荷尔蒙通过去除碳水化合物发挥的生物学功能 从单个亚基通过低聚糖氨基水解酶或 通过在小牛脑垂体片中合成脱糖基牛黄体激素 衣霉素的存在。从这项工作中开发的信息将有助于 为人类黄体生成素的研究设计实验，这将增加我们的 对人类生育规律的理解。

Gonadotropins (LH, FSH and hCG) produced by pituitary and placenta, are key hormones involved in human reproduction. This proposal related to the structure, function and biosynthesis of bLH is a continuation of our ongoing research. The carbohydrate structure in bLH is unique in having a 54-0 substituted N-acetylgalactosamine at the nonreducing end of the asparagine-linked carbohydrate units. The identity of the substituent has not been established. The specific objects of this proposal are: (1) Identification of the substituent on N-acetylgalacosamine. (2) Characterization of the enzymes which incorporate the substituent and N-acetylgalactosamine. (3) Study of the mechanism of assembly of the oligosaccharide-lipid involved in the biosynthesis of bLH carbohydrate by the isolation of major intermediates. (4) Study of the processing of the oligosacchardie after its transfer to the protein by the isolation of various intermediates and the addition of peripheral sugars. (5) Attempt on the total in vitro synthesis of bLH using deglycosylated bLH-Alpha and bLH-Beta, transfer of the oligosaccharide from oligosacharide-lipid intermediate followed by the complete processing of the oligosaccharide. (6) Study of the effect of steroids, LH-releasing factor and cAMP on the synthesis and secretion of bLH. (7) Study of the role of carbohydrate in the biological function of the hormone by the removal of the carbohydrate from the individual subunits by an enzyme oligosaccharide amidohydrolase or by the synthesis of the deglycosylated bLH in calf pituitary slices in the presence of tunicamycin. Information developed from this work will help in designing experiments for the study of human LH, which will add to our understanding of the regulation in human fertility.