STUDIES ON THE CONTROL OF CELL DIVISION IN YEAST

我正在研究细胞分裂的控制，我的具体目标是 相关基因的鉴定，其基因的阐明 分子产物，并最终测定生理 它们所扮演的角色。酵母菌(酿酒酵母)，一种简单的 兼具遗传和生化特性的真核生物 调查，将被用于拟议的研究。在酵母中， 分裂通常是为了响应营养限制而控制的 准备接合。这两种控制形式都集成在 细胞周期中被称为开始的一个阶段的G1间期。毫无疑问，有很多 基因产品直接涉及和逃避其个体 角色将是阐明如何控制细胞分裂的重要一步。 真核生物是有组织的。为了鉴定相关基因，突变体 它们不能完成启动步骤。到目前为止，我已经孤立了 40个独立突变，并将它们分配到四个非连锁互补 组。这些基因现在已经通过重组DNA进行了物理分离 技术，并可用作试剂在表征 它们编码的相应的mRNA和多肽。抗血清反应性 将使用新的嵌合基因来制备针对这些多肽的 该基因可在大肠杆菌中高效表达。细胞内 启动基因的定位、代谢和生理作用 将对产品进行调查。

I am investigating the control of cell division, my specific goals being the identification of the genes involved, the elucidation of their molecular products, and ultimately the determination of the physiological roles that these play. Yeast (Saccharomyces cerevisiae), a simple eukaryotic organism amenable to both genetic and biochemical investigations, will be employed for the proposed research. In yeast, division is normally controlled in response to nutrient limitation and in preparation for conjugation. Both forms of control are integrated in the G1 interval at a step of the cell cycle known as start. Undoubtedly many gene products are directly involved and eludication of their individual roles would be a major step in claritying how control of cell division in eukaryotes is organized. In order to identify the relevant genes, mutants which cannot complete the start step were sought. To date, I have isolated 40 independent mutations and assigned them to four unlinked complementation groups. These genes have now been isolated physically by recombinant DNA techniques and may be used as reagents in the characterization of the corresponding mRNAs and polypeptides that they encode. Antisera reactive against these polypeptides will be prepared using novel chimeric genes which can be expressed at high level in E. coli. The intracellular location, the metabolism and the physiological roles of the start genes products will be investigated.