NUCLEAR GENES REGULATING TRANSLATION OF ORGANELLES MRNAS

真核细胞依赖于核和核之间的相互作用 正常生长发育所需的细胞器基因组。坐标 这些独立的基因组在病毒形成过程中的表达 线粒体和线粒体的能量传导复合体 叶绿体是必不可少的。我们正在研究 核型和叶绿体基因组在植物生长发育中的协同表达 通过遗传和分子手段实现的光合作用。 一些光合作用蛋白的表达，两者都 叶绿体和核编码，受翻译调控 衣藻叶绿体的生物发生过程 莱恩哈迪尔。我们已经确定了一组核突变的特征 它们缺乏特定叶绿体的翻译 编码的mRNA。我们将克隆编码这些基因的核基因 叶绿体跨国激活子的互补作用 通过DNA依赖转化的光合作用突变体，a 最近在五月份实验室开发的工艺。学习表达方式 这些跨国激活子在叶绿体生物发生过程中的作用 应该为理解核能的作用提供一个基础 调控细胞器基因表达的基因。

Eukaryotic cells rely on the interactions of the nuclear and organeller genomes for normal growth and development. Coordinate expression of these independent genomes during the formation of the energy transducing complexes of the mitochondria and chloroplast is essential. We are studying the interactions of nuclear and chloroplast genomes in the coordinate expression of the photosynthetic apparatus by genetic and molecular means. Expression of a number of photosynthetic proteins, both chloroplast and nuclear encoded, is translationally regulated during the biogenesis of the chloroplast in Chlamydomonas reinhardtii. We have characterized a group of nuclear mutants which are deficient in translation of specific chloroplast encoded mRNAs. We will clone the nuclear genes encoding these chloroplast transnational activators by complementation of the photosynthetic mutants via DNA dependent transformation, a process recently developed in may lab. Studying the expression of these transnational activators during chloroplast biogenesis should provide a basis for understanding the role of nuclear genes in regulating organeller gene expression.