GENETIC ANALYSIS OF POLARITY

两个母体效应突变对早期细胞极性有显著影响 胚胎的位置非常接近， 小的缺陷间隔。 一个突变体，Bicaudal-b（Bic-b），是一个显性突变体， 产双尾卵 另一种突变的雌性纯合子，背侧 (dl)产生的胚胎缺乏所有的腹侧结构。 我们提出了一个 许多关于这些基因组织的遗传和分子研究， 他们的时间和行动方式，以及他们的作用和重要性， 在发育中的卵和胚胎发生过程中极性的建立。 我们计划研究这些基因与其他相关基因的相互作用 在极性决定中。 首先进行转化实验， 研究这些基因的表达是如何控制的，其次是了解 更多关于其功能。 我们还计划分离出针对 针对Bic-B和dl基因产物，这些将用于研究 Bic-b和dl基因的分布和细胞内定位 产品.

Two maternal effect mutations with drastic effects on polarity in early embryos have been mapped very close to one another and both fall into a small deficiency interval. One mutant, Bicaudal-b (Bic-b), is a dominant and lays bicaudal eggs. Females homozygous for the other mutation, dorsal (dl), produce embryos that lack all ventral structures. We propose a number of genetic and molecular studies on the organization of these genes, their time and mode of action, and their role and importance in the establishment of polarity in the developing egg and during embryogenesis. We plan to study the interaction of these genes with other genes involved in polarity decisions. Transformation experiments will be done first to study how the expression of these genes is controled and second to learn more about their function. We also plan to isolate antibodies directed against Bic-B and dl gene products and these will be used to study the distribution and intracellular localization of the Bic-b and dl gene products.