DEVELOPMENT OF NEW AUTOMATED POLARIZED LIGHT MICROSCOPE

偏振光显微镜具有独特的测量潜力 亚微观分子动态非破坏性排列 在活细胞和其他标本中。使用传统的Poll-Scope， 然而，单个图像仅显示那些各向异性结构， 相对于极化具有有限范围的取向 显微镜的轴。此外，快速测量受到限制 显示为均匀的单个图像点或单个区域 双折射或其他形式的光学各向异性，而测量 比较几个图像点需要非常长的时间。 我们建议开发一种新的偏振光显微镜，它将 在样品的测量中结合速度和高分辨率 各向异性，与其方向无关。新车的设计 偏光显微镜是在传统的偏振光学显微镜的基础上增加了两个 基本修改：圆形偏振器将取代线性偏振器 偏振器和两个电光调制器将取代 传统补偿器。摄像机和计算机辅助图像 分析将每隔一次提供一次样品各向异性测量 第二，用于构成视场的图像的所有点。这个 测量过程将产生特别清晰的图像，没有 阴影和背景光覆盖整个视场。这些图像 测量将记录精细的结构和分子组织 在标本的薄光学切片内。高度的空间性和 新极光望远镜的时间分辨率及其易用性 用于获取定量样本记录，同时用于整体 视野，将允许新的调查的动态 活细胞和其他系统的大分子组织。

The polarized light microscope has the unique potential to measure submicroscopic molecular arrangements dynamically and non-destructively in living cells and other specimens. With the traditional pol-scope, however, single images display only those anisotropic structures that have a limited range of orientations with respect to the polarization axes of the microscope. Furthermore, rapid measurements are restricted to a single image point or single area that exhibits uniform birefringence or other form of optical anisotropy, while measurements comparing several image points take an inordinately long time. We propose to develop a new kind of polarized light microscope which will combine speed and high resolution in its measurement of the specimen anisotropy, irrespective of its orientation. The design of the new pol-scope is based on the traditional polarized light microscope with two essential modifications: circular polarizers will replace linear polarizers and two electro-optical modulators will replace the traditional compensator. A video camera and computer assisted image analysis will provide measurements of specimen anisotropy once every second for all points of the image comprising the field of view. The measurement process will result in particularly clear images free of shading and background light over the entire viewing field. The images and measurements will document fine structural and molecular organization within a thin optical section of the specimen. The high spatial and temporal resolution of the new pol-scope together with its' ease of use for obtaining quantitative specimen records, concurrently for the whole field of view, will allow new investigations into the dynamics of macromolecular organization of living cells and other systems.