TRANSCRIPTIONAL STUDIES OF OOGENESIS

利用红头丽蝇（Calliphora erythrocephala）（一种双翅目昆虫）， 已经开发了一种系统，该系统允许分析基因的模式， 在“马赛克”蛋的生产过程中的活动。 使用这个系统， 因此，在卵子发生过程中转录的克隆DNA片段将被 确定和进一步分析，以确定 关于他们的表达。 i）这些基因中是否有 只在卵子发生时转录 ii）为任何一个 这些基因在卵母细胞/卵子内显示出特定的定位？ （三） 不完全加工的转录本， 鸡蛋？ iv）这些基因中的任何一个的转录物是否在 成熟/受精？ 卵母细胞/卵蛋白的鉴定 由显示任何这些特征的基因编码的基因将被尝试。 这些研究应该对分子机制产生有价值的见解 在“马赛克”发育系统中的胚胎学决定。 一个 另外的优点来自于生物体的选择。 C.红头畸形 与黑腹果蝇密切相关，因此任何克隆的基因 确定为参与C中的决定性事件。红头绦虫会 用于筛选D.黑腹菌 如果这 证明是可能的，它们功能的分子遗传学研究可能是 执行。

Using the species Calliphora erythrocephala (a meroistic dipteran insect), a system has been developed which permits analysis of the patterns of gene activity during the production of a "mosaic" egg. Using this system, cloned DNA fragments transcribed during oogenesis will therefore be identified and further analysed with a view to establishing the following features concerning their expression. i) Are any of these genes transcribed exclusively during oogenesis? ii) Do transcripts for any of these genes show specific localization within the oocyte/egg? iii) Are incompletely processed transcripts from any of these genes stored within the egg? iv) Are transcripts from any of these genes activated at maturation/fertilization? Identification of the oocyte/egg proteins encoded by genes showing any of these characteristics would be attempted. These studies should yield valuable insights into the molecular mechanisms of embryological determination in a "mosaic" developmental system. An additional advantage comes from the choice of organism. C. erythrocephala is closely related to Drosophila melanogaster and thus any cloned genes identified as involved in determinative events in C. erythrocephala would be used to try and select ther homologues from D. melanogaster. If this proved possible, molecular genetical studies of their function could be performed.