MEMBRANE DOMAINS AND THE CONTROL OF FERTILIZATION

这些研究的长期目标是了解并尽可能 控制亚微观膜结构域的作用 受精 主要地，将使用两种技术来研究 膜结构域的性质：非辐射荧光 共振能量转移与标记冷冻断裂电子 显微镜 将研究两种生物施肥系统： 海胆和老鼠 我们建议批判性地审视 所涉及的膜中结构域组织的性质 在受精中，这些结构域在受精中的作用，以及 域的集合是否可以被操纵 有利地防止或促进受精。 具体来说，我们将问：1）域的性质是什么？ 存在于受精系统的膜中 （a） 脂质被隔离成域？ B）蛋白质是否被隔离到 域名？ c）结构域存在于孤立的膜囊泡中吗？ d）、 结构域是否在没有蛋白质的情况下出现？ e）什么是 这些领域的规模？ 2)域是否在 受精的生理过程 a）过度活化，B） 顶体反应，c）精卵融合，d）皮质 反应 3)是否可以改变域的集合， 抑制和/或增强受精过程？

The long range goal of these studies is to understand and possibly control the role of submicroscopic membrane domains in fertilization. Principally, two techniques will be used to study the nature of membrane domains: nonradiative fluorescence resonance energy transfer and label-freeze fracture electron microscopy. Two biological fertilization systems will be studied: the sea urchin and the mouse. We propose to critically examine the nature of the domain organization in the membranes involved in fertilization, the role of these domains in fertilization, and whether the ensemble of domains can be manipulated advantageously either to prevent or to promote fertilization. Specifically, we will ask: 1) What are the nature of the domains present in the membranes of the fertilization system? a) Are lipids sequestered into domains? b) Are proteins sequestered into domains? c) Do domains occur in isolated membrane vesicles? d) Do domains occur in the absence of proteins? e) What are the sizes of these domains? 2) Do domains play a role in the physiological processes of fertilization? a) Hyperactivation, b) The acrosome reaction, c) Sperm-egg fusion, d) The cortical reaction. 3) Can the ensemble of domains be altered so as to inhibit and/or enhance the fertilization processes?