STRUCTURAL AND FUNCTIONAL STUDIES--UTERINE SMOOTH MUSCLE

下面的提案概述了我们对细胞间的作用的兴趣 哺乳动物生殖功能控制中的交流。 为卵巢和子宫细胞的调节提供了证据 表面结构称为缝隙结，它涉及到直接 各种小的调节和信息分子的交换 在接触的细胞之间。建立椎弓根的解剖学基础 在分娩过程中子宫平滑肌的协调活动是由于 子宫肌层细胞缝隙连接的出现，促进了电 偶联，从而在分娩期间形成单一的电合体。 本提案中概述的研究旨在提供一种独特的 调控机制分析的实验方法 哺乳动物子宫中的细胞间通讯。该计划的目标是 研究内容如下：(A)研究雌激素和黄体酮对 子宫肌层缝隙连接的体外形成及比较 子宫肌层缝隙连接的通透性 血管平滑肌细胞：(B)检验假设 CAMP和cAMP可调节子宫肌层缝隙连接的通透性 肌醇磷脂信号转导途径；(C)发展 子宫肌层细胞-颗粒细胞共培养体系分析 影响缝隙连接通道的调控机制的差异 渗透率：(D)检验电子致密沉积的假设 与受体介导的缝隙连接膜相关 离开cAMP途径的激活代表了腺苷捕获的钙离子 环化酶衍生的焦磷酸阴离子。因为早产与 超过60%的婴儿死亡，减少这种危险需要研究 关于维持妊娠和开始分娩的因素。结果是 这些研究将有助于更好地理解子宫肌层 在分娩过程中的收缩能力，希望对发育有帮助 有效的酵母菌发酵剂。

The following proposal outlines our interest in the role of intercellular communication in the control of reproductive function in mammals. Evidence is provide for the regulation of ovarian and uterine cell surface structures termed gap junctions which are involved in the direct exchange of a variety of small regulatory and informational molecules between cells in contact. The anatomical basis for the establishment of coordinated uterine smooth muscle activity during labor is due to the appearance of myometrial cell gap junctions which facilitates electrical coupling and thereby a single electrical syncytium during parturition. The studies outlined in this proposal are designed to provide a unique experimental approach to the analysis of the mechanisms regulating intercellular communication in the mammalian uterus. The objective of the research are: (a) To study the action of estrogen and progesterone on the formation of myometrial gap junctions in vitro and compare the permeability properties of myometrial gap junctions with those of vascular smooth muscle cells: (b) to test the hypothesis that the permeability of gap junctions in myometrium can be modulated by cAMP and the inositol phospholipid signal transduction pathways; (c) to develop myometrial cell-granulosa co-culture systems in order to analyze differences in the regulatory mechanisms affecting gap junction channel permeability: (d) to test the hypothesis that electron dense deposits associated with gap junction membrane following receptor mediated activation off the cAMP pathway represents Ca++ trapped by adenylyl cyclase derived pyrophosphate anions. As prematurity is associated with over 60% of all infant deaths, reduction of this hazard requires research on the factors that maintain pregnancy and initiate labor. The results of these studies will lead to a better understanding of uterine myometrial contractility during parturition and hopefully, to the development of effective tocolytic agents.