PLATELET STUDIES IN THROMBOCYTOPENIA

我们建议研究抗体与血小板的结合。 特发性血小板减少性紫癜患者，血小板减少症伴 高血小板结合免疫球蛋白、药物引起的血小板减少症和同种异体免疫 人类白细胞抗原和其他抗原引起的血小板减少症。我们提议： 确定血小板结构中的糖蛋白，这些糖蛋白与 抗体通过放射免疫沉淀相互作用，交叉 免疫电泳法和“Western”印迹电泳法。按顺序 为了更准确地对这些抗体所用的蛋白质进行分类 InterAct，一组在小鼠身上制造的单抗 杂交瘤和其中识别各种蛋白质的血小板将是 制造。这些抗体与之反应的蛋白质将与 结果与临床上有意义的抗体。证明了单克隆体 抗体和具有临床意义的抗体与该抗体反应 蛋白质将通过抑制研究和抗原去除获得 学习。蛋白质的同一性将通过电泳法确定 功能测试中的机动性。Ig G也可能与Fc受体结合。当免疫球蛋白 被分子的Fab部分结合，Fc受体被占据。 我们建议通过抗体的Fc部分来确定这是否 分子。我们建议对它们之间的关系进行定量研究 Fc受体的结合与释放之间的关系 血小板的反应。

We propose to investigate the binding of antibodies to platelets in patients with idiopathic thrombocytopenic purpura, thrombocytopenia with high platelet-bound IgG, drug-induced thrombocytopenia, and alloimmune thrombocytopenia due to HLA and other antigens. We propose to propose to identify the glycoproteins within the platelet structure with which these antibodies interact by radioimmunoprecipitation, crossed immuno-electrophoresis, and electrophoresis with "Western" blot. In order to classify more accurately the proteins with which these antibodies interact, a panel of monoclonal antibodies which are made in mouse hybridomas and which identify various proteins of the platelet will be made. The proteins with which these antibodies react will be compared to results with clinically significant antibodies. Proof that the monoclonal antibody and the clinically significant antibody react with the same protein will be obtained by inhibition studies and by antigen removal studies. The identity of the protein will be determined by electrophoretic mobility on functional tests. IgG may also bind by Fc receptors. When IgG is bound by the Fab portion of the molecule, the Fc receptor is occupied. We propose to determine whether this is by the Fc portion of the antibody molecule. We propose to investigate quantitatively the relationships between the binding of the Fc receptor and the generation of the release reaction of the platelet.