MECHANISMS OF RED CELL AGING - HEMOLYTIC ANEMIA

本项目旨在阐明溶血性贫血的病理生理学。 继发于红细胞代谢缺陷。 我们将详细研究红细胞的糖酵解过程。 利用新开发的技术允许新陈代谢流动到 继续进行，不受代谢障碍的阻碍。这是一种新的方法，可以 对红细胞新陈代谢的研究，这将使糖酵解分析在 细节，在类似于红细胞体内存在的条件下。 我们将详细调查在此过程中发生的生化变化 红细胞在体内老化的过程。我们将通过分析 糖酵解中间体在年龄分级的红细胞中，进行性的 代谢改变的发展。 我们将详细研究糖酵解的两个关键酶-己糖激酶 和丙酮酸激酶。我们将尝试阐明它们的分子结构 并评估同工酶变化在这一过程中所起的作用 酶的分子老化，与这可能导致的可能性 从翻译后修饰。

This project aims at elucidating the pathophysiology of hemolytic anemias secondary to defects in red cell metabolism. We will investigate in detail the glycolytic process of the red cell "in vitro" utilizing newly developed techniques that allow metabolic flow to proceed unimpeded by metabolic block. This is a novel approach to the study of red cell metabolism, that shall permit analysis of glycolysis in detail, in conditions similar to those that exist in the red cell "in vivo". We will investigate in detail the biochemical changes that occur during the process of red cell aging "in vivo". We will examine, through analysis of glycolytic intermediates in age-fractionated red cells, the progressive development of metabolic alterations. We will study in great detail two key enzymes of glycolysis - hexokinase and pyruvate kinase. We will attempt to clarify their molecular structure and to assess the role played by isozymic shifts in the process of molecular aging of enzymes, versus the possibility that this may result from post-translational modifications.