FLUPHENAZINE HPLC-RIA ASSAY DEVELOPMENT AND EVALUATION

低剂量给予氟奋乃静癸酸酯已被证明是一个主要的 血浆水平监测方面的分析化学问题。 利用新开发的高亲和力，特异性抗血清， 我们建议开发一种HPLC-RIA联合方法， 将使我们能够定量低于200 pg/ml。 本参考程序 然后将用于评价直接和溶剂萃取RIA程序 在这个研究所开发的。 最后，大量的样品将被 同时分析上述方法和精神抑制剂 放射受体测定 预期两种或更多种的组合可用于本发明。 这些检测方法的应用可能有助于确定血浆水平的临床疗效 关系。

Fluphenazine decanoate given in low dosage has proven to be a major analytical chemistry problem in terms of plasma level monitoring. Utilizing a newly developed high affinity, specific antisera to fluphenazine we propose to develop an HPLC-RIA combined procedure which will enable us to quantitate less than 200 pg/ml. This reference procedure will then be used to evaluate direct and solvent extraction RIA procedures developed at this institute. Finally a large number of samples will be analyzed simultaneously by the above methods and the neuroleptic radioreceptor assay. It is anticipated that a combination of two or more of these assays may help determine plasma level clinical efficacy relationships.