CLONAL LINES OF THE NERVOUS SYSTEM

脊髓少突胶质细胞形成髓鞘的研究 中枢神经系统是一个复杂的发育过程， 许多中间步骤。程序性髓鞘基因 表达既受遗传因素的调节，又受这些基因固有的因素的影响 细胞，以及细胞所遇到的环境影响 从它们的胚胎学位置迁徙到相当远的地方 从原点到它们的最终功能目标位置。这个项目的目的是 增加我们对外部因素的理解，这些外部因素 参与调节少突胶质细胞的分化和 髓鞘的形成和维持。这项研究将使用几个 解决这一问题的模型系统和检测技术 髓鞘形成整个过程的几个阶段。一键 这些实验的成分是我们观察到的，尽管 培养系统正常地进行髓鞘生成。 与这一进程相比，在许多方面都存在差异。 在活体内。我们的工作假设是，这些差异存在于 许多情况是由于在文化中缺席了重要的 生长因子，并通过重新补充缺失的因子， 赤字是可以纠正的。这些系统的第二个关键特征 而相关的检测方法是它们提供了定量的 信息，通常是在单个少突胶质细胞水平上。它 预计这些数据将有助于我们了解 少突胶质细胞分化，从而提供线索 髓鞘形成、维持和发育的关键过程 在正常和疾病状态下的重新髓鞘形成。

The formation of the myelin sheath by oligodendrocytes in the central nervous system is a complex development process with numerous intermediate steps. Programmed myelin gene expression is regulated both by genetic factors intrinsic to these cells, and by environmental influences encountered as the cells migrate considerable distances from their sites of embryological origin to their final functional target locations. This project aims to increase our understanding of extrinsic factors that are involved in the regulation of oligodendrocyte differentiation and myelin formation and maintenance. The study will use several model systems and assay techniques to address this question at several stages of the overall process of myelinogenesis. One key ingredient in these experiments is our observation that although the culture systems carry out myelinogenesis remarkably normally in many respects, there are differences compared to this process in vivo. Our working hypothesis is that these differences are in many cases due to the absence in the cultures of important growth factor, and that by re-supplying the missing factors, the deficits can be corrected. A second key feature of these systems and the related assays is that they provide quantitative information, often at the level of individual oligodendrocytes. It is expected that these data will contribute to our understanding of oligodendrocyte differentition, thereby providing clues to processes critical to myelin formation, maintenance, and remyelination in both the normal and diseased state.