CELL BIOLOGY/IMMUNOBIOLOGY ON ANIMAL MODELS FOR VITILIGO

本文提出的研究的长期目标是促进 知识的病理过程发生在白癜风，一种疾病， 皮肤和眼睛的色素细胞被破坏。 实验 旨在分析两种动物模型中色素脱失的基础 白癜风：广泛研究的史密斯鸡（以前提到的 作为DAM鸡）和新的哺乳动物鼠模型（vi/vi）。 两 所采取的方法涉及基本的黑色素细胞缺陷和 随后自身免疫反应 猪和免疫者 在任何治疗过程中，系统都必须协调一致地操作， 方案，可能表明拟议的研究结果。 这些实验包括对史密斯线黑化的深入研究， 在脉络膜黑素细胞和神经管衍生的黑素细胞中 在培养物中发生功能障碍的方式类似于 体内黑素细胞。 异常黑化与 黑素体的选择性自噬作用也将 研究了 来自在各种不同时间段获得的史密斯鸡的血清 白癜风的发展阶段，将筛选 黑素细胞特异性抗体。 这些抗体将用于 电子免疫细胞化学定位黑素细胞特异性抗原 显微镜 皮肤与脉络膜黑素细胞特异性 将通过建立表面抗原的纯培养物来启动表面抗原。 各自的黑素细胞类型。 此外，毛发的形态分析 将启动vi/vi小鼠的眼球和眼睛脱色， 筛选血清中的抗黑素细胞因子， 灰色突变株系

The long-term objective of the research proposed here is to advance knowledge of the pathologic processes occurring in vitiligo, a disease in which pigment cells of the skin and eyes are destroyed. The experiments are aimed at analyzing the basis for depigmentation in two animal models for vitiligo: the extensively studied Smyth chicken (previously referred to as the DAM chicken) and a new mammalian, murine model (vi/vi). The two approaches to be taken concern the basic, melanocytic defect and the subsequent, autoimmune response. Both the pigmentary and the immune systems will have to be manipulated in concert during any therapeutic regimen that might be indicated by the results of the proposed research. The experiments include in-depth studies of melanization in the Smyth line, both within choroidal melanocytes and in neural-tube derived melanocytes that malfunction in culture in a manner similar to that observed in melanocytes in vivo. The causal relationship between aberrant melanization and the selective autophagocytosis of melanosomes will also be investigated. Serum from Smyth chickens obtained during various developmental stages of the vitiligo, will be screened for melanocyte-specific antibodies. Such antibodies will then be used to localize the melanocyte-specific antigens immunocytochemically by electron microscopy. A search for cutaneous versus choroidal melanocyte-specific surface antigens will be initiated by establishing pure cultures of the respective melanocyte types. In addition, morphologic analysis of hair bulb and eye depigmentation in the vi/vi mouse will be initiated as well as screening for anti-melanocyte factors in the serum of this prematurely graying mutant line.