GENETICS OF HEDGEHOG & ADJACENT LOCI OF DROSOPHILA

这项工作将研究基因在规范中所起的作用 黑腹果蝇胚胎的模式。这 将涉及刺猬的特征，特别是刺猬 基因座位，这是一个节段性的极性类座位。这部作品 将表征空间和时间要求 刺猬基因，胚胎和胚胎后，通过使用 遗传马赛克和温度敏感突变。这 这项工作还表征了分子的结构 Hedgehog基因与RNA的时空表达 这个位置的文字记录。此外，这项工作将 从遗传学的角度描述人类基因组周围的染色体区域 刺猬基因，已知包含另外两个 图案规范位置。 对这个基因和其他基因如何破坏正常的理解 果蝇胚胎的模式将提供更多关于 基因在发育中的有机体的胚胎发生中所起的作用， 并可能适用于对人类发展的理解。 这项工作还将为深入了解基因的机制提供帮助。 法规，因此提供了有价值的信息 了解那些疾病的过程，例如癌症，在这些过程中 某些基因的异常调节可能导致一种病理性的 有问题。

This work will investigate the role genes play in the specification of the pattern of the embryo of Drosophila melanogaster. This will involve the characterization, in particular, of the hedgehog gene locus, which is a segmental polarity class locus. This work will characterize the spatial and temporal requirements for the hedgehog gene, both embryonically and post-embryonically, by use of genetic mosaics and temperature sensitive mutations. This work also characterize the molecular architecture of the hedgehog locus and the spatial and temporal expression of RNA transcripts from this locus. In addition, this work will characterize genetically the chromosomal region around the hedgehog locus, which is known already to contain another two pattern specification loci. The understanding of how this gene and others disrupt the normal pattern of the Drosophila embryo will provide more insight into the role genes play in the embryogenesis of developing organisms, and may be applicable to an understanding of human development. This work will also provide insight into the mechanisms of gene regulation and therefore provide information valuable to understanding those disease processes, such as cancer, in which aberrant regulation of certain genes may lead to a pathological problem.