IMMUNOASSAYS FOR SAXITOXINS

石房蛤毒素是神经毒素， 已知的非蛋白质毒素 有几千例 麻痹性贝类中毒的案例- 死亡率接近10% 因为没有解药 中毒，避免污染的材料是唯一的手段 控制力 目前公认的检测石房蛤毒素的方法 和其天然衍生物是通过小鼠生物测定。 替代 方法已经被寻求，但每一个都有一个或多个重要的 缺点 免疫分析似乎是最有前途的方法 迄今 目前对免疫测定的限制是缺乏能力 检测石房蛤毒素组中的所有毒素， 样品基质中组分的干扰。 方法 用于产生多克隆和单克隆抗体 这将能够检测所有的石房蛤毒素。 一期将 重点研究了两种不同的新蛤蚌毒素半抗原的制备 以及在小鼠中证明免疫应答，或 家兔 第二阶段将延长工作，并最终在 开发合适的非放射性免疫测定。 在 除了用于石房蛤毒素免疫测定外， 所产生的可作为治疗剂具有另外的应用 麻痹性贝类中毒

The saxitoxins are neurotoxins and are among the most potent non-protein toxins known. There are several thousand cases of paralytic shellfish poisoning worldwide each year with a case- fatality rate approaching 10%. Since there is no antidote for such poisoning, avoidance of contaminated material is the only means of control. The currently accepted method of detecting saxitoxin and its natural derivatives is by mouse bioassay. Alternative methods have been sought, but each has one or more significant drawback. Immunoassays appear to be the most promising method to date. Current limitations on immunoassays are lack of ability to detect all toxins in the saxitoxin group and problems with interference due to components in the sample matrix. Methods are proposed for generating polyclonal and monoclonal antibodies which will enable detection of all of the saxitoxins. Phase I will focus on the preparation of two different neosaxitoxin haptens and the demonstration of an immunological response in mice or rabbits. Phase II will extend the work and culminate in the development of suitable non-radioactive immunoassays. In addition to use in saxitoxin immunoassays, the antibodies produced may have additional applications as therapeutic agents in cases of paralytic shellfish poisoning.