SOLID-PHASE ASSAY FOR SALIVA

这项研究的长期目标是开发一种设备， 结合和分析简单，快速，可靠，准确， 高灵敏度测量人类唾液中的化合物。 第一阶段可行性研究的重点是发展固体 阶段测定部分，孕酮作为原型分析物。 孕酮测定的主要应用将是用于 妇女生育状况的评估（卵泡期与黄体期 月经周期与怀孕）。 开发的技术 还将提供 使测定适应于其它生物介质（例如马， 牛和猪的唾液）以促进经济家畜 繁殖，并与不同的抗体，以检测其他 物质. 最初的试验将利用现有的，高度 孕酮特异性单克隆抗体。 当用于 固相测定，该抗体已被证明能准确地 血浆中孕酮的估计值。 述系统被 将使用放射性标记的 指示剂，但高灵敏度的替代品，用于比色测定 将探讨是否符合相同的质量标准， 在进展允许的情况下并行执行。 独特的 在第二阶段开发的收集/测定装置设计为 去除食物颗粒，消除内源性问题 唾液粘蛋白，并迅速产生易于可视化的比色 差异 一旦方法的可行性得到证明， 将开展项目，使技术适应 定量其它化合物和试剂（例如街头毒品， 皮质激素、毒素和治疗药物）。

The long range goal of this research is to develop a device incorporating and assay for simple, quick, reliable, accurate, and highly sensitive measurements of compounds in human saliva. Phase I feasibility research is focused on development of the solid phase assay portion with progesterone as the prototypic analyte. The primary application of the progesterone assay will be for the assessment of fertility status in women (follicular vs. luteal phase of the menstrual cycle vs. pregnancy). Technologies resulting from development of the proposed product will also provide the basis for adapting the assay to other biological media (e.g. equine, bovine, and porcine saliva) to facilitate economic domestic animal breeding and, with different antibodies, to detect other substances. The initial assay will utilize an existing, highly specific monoclonal antibody to progesterone. When used in a solid phase assay, this antibody has proven to give accuate esimates of progesterone in blood plasma. The system being proposed will be evaluated and optimized with radiolabeled indicator but highly sensitive alternatives for a colorimetric assay meeting the same quality criteria will be explored and implemented in parallel as progress allows. The unique collection/assay device to be developed in Phase II is designed to remove food particles, eliminate problems with endogenous salivary mucins, and rapidly yield readily visualizable colorimetric differences. Once feasibility of the approach is proven, separate projects will be undertaken to adapt the technology to quantitation of other compounds and agents (e.g. street drugs, corticoids, toxins and therapeutic drugs).