HIGH PERFORMANCE IMMUNOAFFINITY CHROMATOGRAPHY SUPPORTS

生产治疗上有用的药物的主要挑战之一是 蛋白质，包括重组DNA技术的产物， 蛋白质的快速和成本有效的纯化。 高 高效免疫亲和色谱是一种功能强大的新型 用于生产规模蛋白质纯化的技术。 使用 这种技术受到费用和困难的阻碍， 将特异性抗体与二氧化硅载体定制偶联。 在 除了对生产规模的活化柱的需求之外， 基础研究人员表示迫切需要这种类型的 活化的二氧化硅载体以较小的形式。 该提案概述了活性二氧化硅载体的发展 将其预填充到柱中并用于固定化 客户提供的特异性抗体。 第一阶段包括 一种新型活性二氧化硅的制备方法 载体，肼HiPAC，和使用该载体来制备 IgG通过抗体上的寡糖。 在第二阶段， 用于将IgG固定在预填充有活化的 将开发硅。

One of the major challenges in production of therapeutically useful proteins, including products of recombinant DNA technology, is rapid and cost-effective purification of the proteins. High Performance Immuno-Affinity Chromatography is a powerful new technique for protein purification at production scale. Use of this technique has been hampered by the expense and difficulty of custom coupling specific antibodies to silica supports. In addition to the demand for production-scale activated columns, basic researchers have expressed an urgent need for this type of activated silica support in a smaller format. This proposal outlines development of activated silica supports which will be pre-packed into columns and used for immobilization of specific antibodies by the customer. Phase I includes development of protocol for preparation of a novel activated silica support, hydrazine HiPAC, and use of this support to immobilize IgG via oligosaccharides on the antibody. In Phase II, protocols for immobilization of IgG on columns pre-packed with activated silica will be developed.