MALE GERM CELL DEVELOPMENT IN TRANSGENIC MICE

成年雄性动物成熟生殖细胞的形成是 独特的发展体系。 精子发生需要 维持能够自我更新的干细胞库， 经历复杂的分化过程形成单倍体 雄配子 将新基因引入小鼠体内， 研究这一复杂过程的有力方法， 可能揭示新的基因调控机制。 我们提出 四个方面的研究。 1. 为了确定所需的顺式作用调控元件， 小鼠精蛋白-1基因的精子细胞特异性转录， 编码一种独特的蛋白质， 凝 2. 为了确定RNA序列在翻译中的作用， 鱼精蛋白信息的调节和稳定。 3. 为了确定大分子在 合胞精子细胞分化过程中。 4. 分析两个转基因小鼠品系， 男性生殖细胞发育的特征性缺陷。 这些实验代表了一种新的研究方法， 精子发生，并将提供深入了解的过程， 分化和基因调控。

The formation of mature germ cells in the adult male animal is a unique developmental system. Spermatogenesis requires the maintenance of a stem cell pool which can self renew and can undergo a complicated differentiation process to form the haploid male gamete. The introduction of new genes into mice provides a powerful method by which to study this complicated process and may reveal novel mechanisms for gene regulation. We propose studies in four areas. 1. To identify the cis-acting regulatory elements required for spermatid specific transcription of the mouse protamine-1 gene, which codes for a unique protein involved in sperm chromatin condensation. 2. To determine the role of RNA sequences in the translational regulation and stability of protamine message. 3. To establish the degree of macromolecular movement between syncytial spermatids during differentiation. 4. To analyze two transgenic mouse lines which process distinct characteristic defects in male germ cell development. These experiments represent a new approach to the study of spermatogenesis and will provide insight into the process of differentiation and gene regulation.