MALE GERM CELL DEVELOPMENT IN TRANSGENIC MICE

成年雄性动物成熟生殖细胞的形成是一个 独特的发展体系。精子发生需要 维持干细胞库，该干细胞库可以自我更新 经过复杂的分化过程形成单倍体 雄配子。新基因的引入为小鼠提供了一种 研究这一复杂过程的有力手段 可能揭示基因调控的新机制。我们建议 四个方面的研究。 1.确定所需的顺式代理监管要素 小鼠鱼精蛋白-1基因的精子细胞特异性转录， 精子染色质中涉及的一种独特蛋白质的密码是什么 冷凝。 2.确定RNA序列在翻译中的作用 鱼精蛋白信息的调节和稳定性。 3.确定大分子间的运动程度 合胞体精子细胞在分化过程中。 4.分析两个处理不同过程的转基因小鼠系 男性生殖细胞发育中特有的缺陷。 这些实验代表了一种新的研究方法 并将提供对精子发生过程的洞察 分化和基因调控。

The formation of mature germ cells in the adult male animal is a unique developmental system. Spermatogenesis requires the maintenance of a stem cell pool which can self renew and can undergo a complicated differentiation process to form the haploid male gamete. The introduction of new genes into mice provides a powerful method by which to study this complicated process and may reveal novel mechanisms for gene regulation. We propose studies in four areas. 1. To identify the cis-acting regulatory elements required for spermatid specific transcription of the mouse protamine-1 gene, which codes for a unique protein involved in sperm chromatin condensation. 2. To determine the role of RNA sequences in the translational regulation and stability of protamine message. 3. To establish the degree of macromolecular movement between syncytial spermatids during differentiation. 4. To analyze two transgenic mouse lines which process distinct characteristic defects in male germ cell development. These experiments represent a new approach to the study of spermatogenesis and will provide insight into the process of differentiation and gene regulation.