FERTILE PERIOD PREDICTION WITH A TRIF BIOSENSOR

尽管存在几种场外排卵药物，但没有一种药物适用于 避孕应用，因为它们检测到黄体生成素(LH) 仅在排卵前16-36小时出现排卵高峰。这 研究人员将根据这些数据开发一个生育期预测系统 72小时尿雌酮-3-葡萄糖醛酸苷(E1-g)增加 排卵前和孕二醇葡萄糖醛酸苷(PD-g)升高 在排卵之后。E1-g的平均值增加了三倍。 比黄体生成素峰值早两天，但它更缓慢的上升需要更多的 定量检测装置比开或关色OTC进行黄体生成素试验。这 障碍将通过采用一种新的、竞争性的免疫分析来克服- 基于全内反射荧光(TIRF)生物传感器 经过共价键合处理的石英光纤探头 E1-g，并预先与带有荧光的e1-g抗体孵育 分子。当尿中的e1-g与结合的e1-g竞争时，抗体 会迁移到尿样中，从而减少荧光信号。 当传感器在排卵前测量e1-g增加时，它的控制 单位将标志着一个肥沃时期的开始。一个类似的传感器，用于 妊娠二醇葡萄糖醛酸化物将标志着生育期的结束。

Although several OTC ovulation texts exist, none are suitable for contraception applications because they detect luteinizing hormone (LH) which exhibits a peak preceding ovulation by only 16-36 hours. This research would develop a fertile period prediction system based upon the presence of an increase in urinary estrone-3-glucuronide (E1-g) 72 hours prior to ovulation and an increase in pregnanediol glucuronide (Pd-g) following ovulation. The mean value of E1-g rises by a factor of three two days prior to the LH peak, but its more gradual rise requires a more quantitative detection device than the on or off color OTC LH tests. This obstacle would be overcome by employing a novel, competitive, immunoassay- based, total internal reflectance fluorescence (TIRF) biosensor consisting of a quartz fiber probe which had been pretreated with covalently bound E1-g and previously incubated with antibody to E1-g bearing fluorescent molecules. As urinary E1-g competed with the bound E1-g, the antibody would migrate into the urine sample thus reducing the fluorescent signal. When the sensor measures the E1-g increase prior to ovulation, its control unit would signal the beginning of a fertile period. A similar sensor for pregnanediol glucuronide would signal the end of the fertile period.