QUANTITATION OF ESTROGEN RECEPTOR CONCENTRATION IN CELLS

该项目的目标是开发定量雌激素。 使用高分辨率数字技术测量受体浓度 图像显微技术。这些技术将使直接 测量组织和细胞中的受体浓度。这 是对目前生化分析的改进，目前只有 给出一个受体浓度的平均值 均质样样品可能含有不同的 恶性细胞的比例。建议的化验结果将1) 测量细胞群体中受体的浓度，2) 肿瘤群体中含有雌激素的细胞比例 受体在一定浓度水平以上。最近的 抗雌激素受体单抗的研究进展 使免疫细胞化学方法的发展 雌激素受体的定位是可行的。最近的附加内容 定量成像细胞学的进展可以与 从而开发出一种快速、高效、准确的检测方法 并消除了该方法的视觉主观成分。 这些新技术的结合产生了一种新的 定量分析尤其重要，因为它已经 研究表明，肿瘤中含有雌激素受体的患者 对激素治疗有更高的反应概率， 似乎比那些患有肿瘤的患者预后更好 不含这种受体。

The objective of this project is to develop quantitative estrogen receptor concentration measurements using high resolution digital image microscopy techniques. These techniques will enable direct measurements of receptor concentration in tissue and cells. This is an improvement over the current biochemical assays which only give an average value for receptor concentration in a homogenized specimen sample which may contain varying proportions of malignant cells. The assay proposed will 1) measure the concentration of receptors in cell populations, 2) the proportion of cells in a tumor population that have estrogen receptor above a certain concentration level. The recent development of monoclonal antibodies against estrogen receptors makes the development of methods for immunocytochemical localization of estrogen receptors feasible. Additional recent advances in quantitative imaging cytology can be combined with this to develop a fast, efficient and more accurate assay method and to eliminate the visual subjective components of the method. This combination of these new techniques to produce a new quantitative assay is particularly important since it has already been shown that patients whose tumors contain estrogen receptors will have a higher probability of responding to hormonal therapy, and seem to have a better prognosis than patients whose tumors do not contain this receptor.