SIMPLIFIED FROZEN BLOOD METHODOLOGY FOR AUTOLOGOUS BLOOD

这个项目的总体目标是确定冻结的红色 在-25oC下储存长达6个月的细胞将在体内具有 相当于冰冻红细胞在-65℃保存一段时间的存活率 最长达10年，并将符合食品和药物管理局制定的标准 药品监督管理局生物研究和审评办公室(FDA-OBRR) 对于血液产品，红细胞被冷冻。这样做的具体目的是 项目是确定红细胞是否冻结并储存在零下25摄氏度 具有足够的回收率和体外生化特性 证明它们用于输血疗法是合理的，即至少80%的恢复 脱甘油后红细胞2，3，DPG水平正常 至少6760%的基线ATP水平。这些研究是第一部分 一项发展计划，该计划将提供技术来扩大 自体献血者计划的能力，包括使用 一种简化的-25oC冷冻保存及回收方法 适合输血的红血球。这样的一个节目 可能会在社区临终关怀中有广泛的用途和吸引力， 血库，在欠发达地区，那里复杂的冷冻和 在军队中，储存设备并不是现成的 军队中的每一位成员都是潜在的 献血者。

The overall goal of this project is to determine whether frozen red cells stored at -25oC for periods up to six months will have an in vivo survival equal to that of frozen red cells stored at -65oC for periods up to 10 years and will meet the criteria established by the Food and Drug Administration Office of Biological Research and Review (FDA-OBRR) for the blood product Red Blood Cells Frozen. The specific aim of this project is to determine whether red cells frozen and stored at -25oC have sufficient recovery and in vitro biochemical characteristics to justify their use for transfusion therapy, i.e., at least 80% recovery of red cells following deglycerolization, normal 2,3, DPG levels and at least 6760% of the baseline ATP level. These studies are the first part of a development plan that would provide the technology to expand the capabilities of autologous blood donor programs to include the use of a simplified method for the freezing preservation at -25oC, and recovery of red blood cells that are suitable for transfusion. Such a program could potentially have wide use and accptance in community hosptials, blood banks, in underdeveloped areas where sophisticated freezing and storage equipment is not readily available and in the military establishment where every member of the armed forces is a potential blood donor.