QUANTIFICATION OF ONCOGENE PRODUCTS IN CELLS AND TISSUE

本项目的目标是概念化一种测定方法， 定量测定细胞和组织中的癌基因产物。在第一阶段 通过努力，由HER2癌基因编码的蛋白质，P185 HER2，将被 作为一个具体的例子，因为它已经强烈暗示， 乳腺癌的生物学行为，并可能有预后 意义使用的技术将是高分辨率的技术 数字图像显微镜和免疫组织化学定位。以来 免疫组织化学允许分析单个细胞，非常小， 可以使用组织样品，使得该测定能够在小的细胞上进行。 细针抽吸物的病变、活检或细胞学涂片 乳腺肿瘤然而免疫组化的缺点是 技术就是方式。这项研究旨在改善这一点。 限制.所提出的分析方法，依靠数字图像， 处理，将是一个定量测定癌基因表达， 单个细胞，可以很容易地和快速地进行 样本样本的恶性细胞。

The objective of this project is to conceptualize an assay method to quantitate - oncogene products in cells and tissues. In the first Phase I efforts, the protein encoded by the HER2 oncogene, P185 HER2, will be used as a specific' example, since it has been strongly implicated in the biological behavior of breast cancer and may have prognostic significance. The techniques used will be those of high resolution digital image microscopy and immunohistochemical localization. Since immunohistochemistry permits analysis of individula cells, very small tissue samples can be used, enabling this assay to be performed on small lesions, biopsies or cytological smears of fine needle aspirates of breast tumors. However the disadvantage of immunohistochemical techniques is the manner. This research is aimed at improving this limitation. The proposed assay method, relying on digital image processing, will be a quantitative assay for oncogen expression in individual cells which can be performed easily and quickly on the malignant cells of a specimen sample.