SURFACE-MODIFICATION OF BIOMATERIALS

所有与血液接触的合成材料都会遇到血液问题 兼容性。血浆蛋白质的快速吸附以及随后的 血小板的黏附和激活会导致血栓的形成。什么时候 患者植入血管装置或进行血液透析 程序中，必须给出抗凝剂的维持水平，以防止 危及生命的血栓形成。然而，这些药物具有很大的危险性 副作用。非血栓形成装置聚合物的制备将减少 需要如此高水平的这些药物。对于这个项目，我们建议 利用光化学偶联剂对生物医学装置进行表面修饰 预防血栓形成的材料。亲水性材料将是 固定化以防止血浆蛋白沉积，并将肝素 连接到这些间隔物的末端以提供表面抗凝剂 活动。选择了光化学，因为这种化学允许普通 与多种医用设备聚合物偶联。我们会做好准备 几种光活化试剂，然后展示光固定化 这些试剂与相关材料有关。接下来，体外抗凝剂和 将进行血小板黏附和激活试验，以评估 改性表面的效率。这些体外分析将导致 额外的化学优化和体外分流试验 II期部分的血栓形成能力和一般血液相容性 这个项目的。

All synthetic materials which contact blood experience problems with blood compatibility. The rapid adsorption of plasma proteins and subsequent adherence and activation of platelets leads to thrombus formation. When patients are implanted with vascular devices or undergo hemodialysis procedures, maintenance levels of anticoagulants must be given to prevent life-threatening clot formations. However, these drugs have very dangerous side effects. Preparation of non-thrombogenic device polymers would reduce the need for such high levels of these drugs. For this project we propose to use photochemical coupling reagents to surface-modify biomedical device materials for thrombosis prevention. Hydrophilic materials will be immobilized to prevent plasma protein deposition, and heparin will be attached to the ends of these spacers to give the surface anticoagulant activity. Photochemistry is chosen, since this chemistry allows generic coupling to a wide range of medial device polymers. We will prepare several photoactivatible reagents, then demonstrate photoimmobilization of these reagents to relevant materials. Next, in vitro anticoagulant and platelet adherence and activation assays will be conducted to evaluate the efficacies of the modified surfaces. These in vitro analyses will lead to additional chemistry optimization and ex vivo shunt testing of thrombogenicity and general hemacompatibility during the Phase II portion of this project.