13 C - LABELED TRIOLEIN FOR DIAGNOSTIC BREATH TESTS

~(14)C-三油酸呼气测试是经典72-碳三烯呼气测试的流行替代品。 HR粪便脂肪采集法测定脂肪吸收不良 简单性和可以做出诊断的快速。然而， 因为对低水平辐射的长期影响的担忧 (尤其是在摄入时)，这项测试通常不用于儿童或 育龄妇女。放射性碳的替代 它的稳定同位素(~(13)C)及其同位素比质量法检测 光谱仪，现已向任何人提供三油酸呼气测试 受试者的数量。目前使用它的一个主要限制是 13C标记的三油酸甘油酯成本较高。 我们提出了一种生物合成三油酸脂的方法 将导致标签的含量增加18倍。已过标签 (100%掺入)~(13)C-三油酸脂将由自养产生 以~(13)CO_2为碳源生长一株高产油酸微藻 唯一的碳源。我们希望这项技术是在 这笔赠款将降低制作成本，并增强对 这种化合物使得每名患者的测试成本降低了10% 意识到了。通过降低测试成本和消除摄取 放射性化合物，三油酸呼气测试应该更容易 可供临床医生用于妇女和儿童的诊断。

The 14C-triolein breath test is a popular alternative to the classical 72- hr fecal fat collection for measuring fat malabsorption because of its simplicity, and the rapidity with which a diagnosis can be made. However, because of the concern for the long term effects of low-level radiation (especially when ingested), this test is generally not used in children or women of child-bearing age. The replacement of the radioactive carbon with its stable isotope (13C) and its detection using an isotope ratio mass spectrometer, has now made the triolein breath test available to any population of subjects. A principal limitation in its use today is the high cost of 13C-labeled triolein. We are proposing a biological approach to the synthesis of triolein which will result in an 18-fold increase in the content of the label. Perlabeled (100% incorporation) 13C-triolein will be produced by the autotrophic growth of a high-oleic oil producing strain of microalgae using 13CO2 as the sole carbon source. It is our hope that the technology developed in this grant will reduce the production costs and enhance the sensitivity of this compound such that a 10-fold reduction in cost per patient test can be realized. By reducing this test cost and eliminating the ingestion of radioactive compounds, the triolein breath test should be more readily available to the clinician for diagnostic uses in women and children.