OXYGEN BIO-ANALYZER USING OPTICAL TECHNIQUE

这笔赠款的目标是开发光学传感系统，用于 用培养的真核细胞进行体外代谢检测 细胞。该技术可以快速原位分析多个细胞样本 在小的培养器皿中。该方法具有一定的实用价值 生物检测，如细胞毒性研究、药物敏感性测试、细胞 激活和增殖测试。 这种创新的方法利用了放入化验容器中的指示剂。 其由光学读取器询问。因此，它是切实可行的 测量由于细胞新陈代谢引起的样品微环境的变化。 差异可作为细胞死亡、增殖、 激活或抑制。 第二阶段的目标是开发在 不同形态的细胞培养血管和进化出的各种读物 用于光学分析化验血管的仪器。这项研究 目的重点研究小样本、动力学分析的特点 研究、持续监控、经济、快速和多功能性。这个 生产的技术将产生满足多样化需求的产品系列 应用驱动的研究、医药和工业对细胞的需求 生长和抑制生物测定。

The objective of this grant is to develop optical-sensing systems for measuring in vitro metabolism-based assays with cultured eukaryotic cells. The technique can rapidly analyze in situ multiple cell samples in small culture vessels. The approach is valuable for conducting bioassays in such as cytotoxicity studies, drug sensitivity testing, cell activation and proliferation testing. The innovative approach utilizes an indicator put into assay vessels which is interrogated by an optical reader. It is thereby practical to measure changes in the sample microenvironment due to cell's metabolism. Differences serve as bioassay indices of cell death, proliferation, activation or inhibition. The Phase II aims are to develop the technology for performing assays in different forms of cell culture vessels and to evolve various reading instruments for optically analyzing the assay vessels. The research objectives focus on the features of assaying small samples, kinetic studies, continuous monitoring, economy, speed, and versatility. The technology produced will engender a family of products meeting diverse application-driven needs in research, medicine and industry for cell growth and inhibition bioassays.