HIGH-MASS MOLECULAR ION ELECTROHYDRODYNAMIC EMISSION

本申请的长期目标是开发 生物大分子质量分析仪器 质量范围为10，000至100，000 amu。 阶段的具体目标 二是演示生物的排放和检测 样品离子到MW 11，500使用电流体动力学（EHD） 安装在JEOL双聚焦质量上的电离源 光谱仪 实现这一目标的方法包括 继续进行I期研究， 去溶剂化、溶剂化学和处理小的和 有价值的样本 分子离子强度的增强 通过冷却EHD离子源和改进的离子发射 将寻求通过添加光束光学器件进行传输。 特别 将把注意力集中在EHD的验证能力上 电离过程，以产生双电荷和三电荷离子， 扩展了样品离子的可检测质量范围。 水性或 将探索和表征高挥发性溶剂， 它们实现完全样品去溶剂化的潜力， 降低了用离子质谱仪观察到的高质量离子谱的复杂性， 甘油 EHD离子源的商业应用 开发，再加上匹配的质量分析仪，将影响 生物技术领域通过引入一种新的，更准确的方法， 蛋白质和其它高分子量物质的分子量测定 大分子生物化合物

The long term objective of this application is to develop instrumentation for the mass analysis of biological macromolecules in the mass range 10,000 to 100,000 amu. The specific aim of Phase II is to demonstrate the emission and detection of biological sample ions to MW 11,500 using an electrohydrodynamic (EHD) ionization source mounted on a JEOL double focusing mass spectrometer. The methodology for meeting this goal includes a continuation of Phase I studies which examined sample ion desolvation, solvent chemistry and methods for handling small and valuable samples. Enhancement of the intensity of molecular ion emission by cooling the EHD ion source and improved ion transmission by adding beam optics will be pursued. Particular attention will be focused on the demonstrated capability of the EHD ionization process to produce doubly and triply charged ions for extending the detectable mass range of sample ions. Aqueous or high volatility solvents will be explored and characterized for their potential of achieving complete sample desolvation and reducing the complexities in high mass ion spectra observed with glycerol. The commercial application of EHD ion source development, coupled to a matching mass analyzer, will impact the biotechnology field by introducing a new, more accurate method of high mass molecular weight determinations for proteins and other macromolecular biological compounds.