PURCHASE OF PROTEIN SEQUENCER AND PEPTIDE SYNTHESIZER

需要资金购买一台气相蛋白质测序仪和一台高 与高效液相色谱分析系统对接 它。高效液相色谱系统用于鉴定 在每一个循环中释放的氨基酸的PTH-衍生物 埃德曼的连续退化。这个系统必须是优秀的 可靠性，因为可以使其跟上 只要它不出故障，就可以使用定序器。该设备将被设置为 在E.Margoliash博士的实验室里，他长期和广泛地 在蛋白质测序的各个阶段都有经验，并会指导顺利 设施的运行情况。它将由L.G.博士直接监督。 全副武装，由一名称职的技术人员管理。 还要求提供资金用于购买多肽合成器， 分析型和制备型高效液相色谱 用于合成产品提纯的系统和 相关项目。该设备将安装在G博士的位置并由其监督。 Flouret在多肽方面拥有广泛而长期的背景 综合，在该领域整个发展时期，自 上世纪60年代初的S。他将得到一名称职的技术人员的协助。 两个技术员的工资以及两个技术员的维修费 西北大学已承诺建立分析和合成设施 所要求的大学长期持续运营 仪器仪表。由五名成员组成的咨询委员会，包括校长 已经成立了一个调查员来监督这两个设施的运作， 维护共享安排的适当组织，并监督其 根据主要用户群体的研究需要进行绩效评估； 由14名调查人员组成。预计所请求的 设施，定期和最小的延误，如果有的话，将 提供蛋白质测序和多肽合成的要求 主要用户群体的研究项目。如果，在这些之外 要求，设备运行时间将可用，它意在 为大学的其他研究人员提供服务，他们可能偶尔会有氨基 酸测序和多肽合成的需要。

Funds are requested to purchase a gas phase protein sequencer and a high performance liquid chromatography analytical system to be interfaced with it. The HPLC system is intended for the identification of the PTH-derivatives of the amino acids liberated at each cycle of the sequential Edman degradation. This system has to be of excellent reliability since it can be made to keep up with the output of the sequencer as long as it does not break down. This equipment will be set up in the laboratory of Dr. E. Margoliash who has had long term and extensive experience in all phases of protein sequencing, and will direct the smooth operation of the facility. It will be supervised directly by Dr. L. G. Armes and run by a competent technician. Funds are also requested for the purchase of a peptide synthesizer, analytical and preparative scale high performance liquid chromatography systems to be used in the purification of the synthetic products and related items. This equipment will be located in and supervised by Dr. G. Flouret who has an extensive and longstanding background in peptide synthesis, over the entire period of the development of that field since the early 1960's. He will be assisted by a competent technician. The salaries of both technicians, as well as funds for maintenance of both analytical and synthetic facilities have been committed by Northwestern University for continued long term operation of the requested instrumentation. A five-member advisory committee, including the principal investigator, has been formed to oversee the operation of both facilities, maintain proper organization of the sharing arrangements and monitor their performance in the light of the research needs of the major user group, consisting of 14 investigators. It is expected that the requested facilities, on a regular basis and with minimal, if any, delay, will provide for the protein sequencing and peptide synthesis requirements of the research projects of the major user group. If, beyond these requirements, equipment operating time will be available, it is intended to service other investigators at the University who may have occasional amino acid sequencing and peptide synthesis needs.