Live cell super-resolution microscopy assessment of novel ratiometric luminescent transition metal complexes.

该提案旨在加强申请人和泰国Phitsanulok Naresuan大学的Filip Kielar博士之间目前正在进行的合作，并提供至关重要的下一个里程碑。此次合作基于Filip Kielar博士在过渡金属络合物领域的专业知识，以及Robert Pal博士在光学显微镜和相关的活细胞成像和时间分辨光谱学方面的专业知识。由于过渡金属络合物的独特性质，例如相对于短寿命的组织自体荧光，它们具有更长的荧光寿命，以及它们作为光诱导单线态氧发生器的潜在应用，它们在纤维素中的应用引起了人们的极大兴趣。我们建议使用各种光学技术，如激光扫描共聚焦、时间分辨和荧光寿命成像显微镜，研究这些新合成的化合物，这些化合物可以报告活细胞研究中的关键生物重要分析物。这种合作最初是2014年皇家学会国际交流计划授予Pal博士和Kielar博士的赠款的结果，允许他们参与合作。该计划是一项仅限旅行的拨款，允许他们每人进行为期两周的访问，促进至关重要的面对面会议和讨论，以形成和推动项目向前发展，最大限度地提高科学产出，并提供研究生和研究生讲座。整整一年后，经过大量的艰苦工作，我们幸运地处于这样一个位置，我们的集体发现发表在了道尔顿会刊(17420，45,2016)上。由于这一良好的合作，泰国研究基金在12/2016年度提交了更广泛的赠款，以促进将在Durham研究的下一代建筑群的合成(授予Kielar博士，于2017年02/2开始，不包括PI的旅行津贴)。然而，上述拨款仅用于为那瑞宣大学的研究人员(3年制博士)和消耗品费用提供便利。Pal博士已经将这个项目的继续作为2017/18年的专门硕士学位。Narressuan大学的研究人员对我们在这一主题上的初步合作和富有成效的沟通感到非常高兴，并坚信，由于达勒姆大学的专业知识和奉献精神，我们在如此短的时间内在这一主题上取得了如此多的成就，尽管我们的物流源于非常不同的地质和财务状况。

This proposal is aimed at strengthening and providing the all-important next milestone in the current ongoing collaboration between the applicant and Dr. Filip Kielar, Naresuan University, Phitsanulok, Thailand. The collaboration is based on the Dr. Filip Kielar's expertise in the field of transition metal complexes and the expertise of Dr. Robert Pal in optical microscopy and associated live cell imaging and time-resolved spectroscopy. Due to the unique nature of transition metal complexes, such as longer fluorescent lifetime as opposed to short lived tissue auto-fluorescence and their potential application as photoinduced singlet oxygen generators, their in cellulo application is of great interest. We propose to study these newly synthesized compounds that can report on key biologically important analytes in live-cell studies using a variety of optical techniques, such as Laser Scanning Confocal-, Time-resolved- and Fluorescent Life Time imaging Microscopy.This collaboration was initially established as a result of a Royal Society International Exchange Scheme grant in 2014 that was awarded to Dr. Pal and Dr Kielar allowing them to engage in collaborative work. The scheme, being a travel only grant, allowed them to conduct a 2 week long visit each, facilitating all-important face to face meetings and discussions to shape and drive the project forward maximising scientific output and delivering under and post graduate lectures. Exactly one year and lots of hard work later we were in the fortunate positon, that our collective findings have been published in Dalton Transactions (2016, 45, 17420). As a result of this well-oiled collaboration a further more extensive Thai Research Fund grant was submitted in 12/2016 to facilitate the synthesis of the next generation of complexes to be studied at Durham (awarded to Dr. Kielar, commenced 02/2017 with no travel allowance for the PI). However, the above mentioned grant only facilitates research staff (3 year PhD) and consumables cost to be met at Naresuan University. Dr. Pal has already earmarked the continuation of this project as a dedicated 2017/18 MSc. student.Researchers at Narressuan University are extremely happy with our preliminary fruitful collaborative work and communications on this subject, and truly believe that due to the expertise and dedication at Durham we have achieved so much in so little time on this subject despite the logistics originating from our very different geological and financial position.