DYNAMICS OF SPERM MEMBRANES, FERTILIZATION AND EMBRYOS

长期目标是更好地理解 哺乳动物受精。分子科学中多种工具的发展 生物学与精子体外保存的新兴技术 细胞、卵母细胞和最终的体外受精增加了机会 分离和鉴定关键的形态、生化和 对正常脑血管形成和发展至关重要的生理变化 概念图。 具体地说，本研究的目的是：(1)表征 精子膜脂类成分的变化与 获能和顶体反应；(2)改进现有的 体外受精；(3)录像带和分析通过 体外动态事件的计算机增强图像分析 施肥；以及最后(4)将这一知识和技术推广到 受精后早期发育的研究。 以前在这一领域的许多研究都涉及一系列 孤立的观察。通过结合生化和生物化学技术 在控制良好的系统中不同阶段的超微结构分析，以及 本研究持续记录与受精有关的变化 应该有助于解释和整合动态事件 与受精有关。这些技术的发展将 (1)促进发现各种环境的时间和方式的机会 影响早期发育的条件和(2)直接改善 人类和动物的体外受精过程。

The long term objective is to better understand the dynamic process of mammalian fertilization. The development of many tools in molecular biology and the emerging technologies for in vitro maintenance of sperm cells, oocytes and finally fertilization in vitro enhances the opportunity to isolate and identify critical morphological, biochemical and physiological changes vital to the formation and development of a normal conceptus. Specifically, the objectives of this research are: (1) to characterize changes in lipid composition of sperm call membranes associated with capacitation and the acrosome reaction; (2) to improve existing systems of in vitro fertilization; (3) to record on video tape and analyze through computer-enhanced image analysis the dynamic events surrounding in vitro fertilization; and finally (4) to extend this knowledge and technology to the study of early post-fertilization development. Much of the previous research in this area has involved a series of isolated observations. By combining the techniques of biochemical and ultrastructural analysis at various stages in well-controlled systems, and continuously recording changes associated with fertilization this research should be helpful in interpreting and integrating the dynamic events associated with fertilization. The development of these techniques will (1) facilitate the opportunity to detect when and how various environmental conditions impinge on early development and (2) directly improve the process of in vitro fertilization in humans and in animals.