DESATURATION OF ESSENTIAL FATTY ACIDS USING STABLE ISOTOPE/MASS SPECTROMETRY

去饱和是必需脂肪酸改性的必要步骤 从亚油酸和亚麻酸到花生四烯酸和二十二碳六烯酸， 分别 这些脂肪酸的水平被乙醇降低 消耗血细胞、肝脏和大脑。 的作用机制 这些组织中必需脂肪酸水平的破坏主要是 未知 这种酒精引起的缺乏症的一种可能解释是 抑制18-碳必需脂肪酸的延长/去饱和 酒精 稳定同位素气相色谱/质谱（GC/MS） 已经开发了一种方法，允许在体内检查这些 流程. 该技术提高了方法的灵敏度， 几个数量级，使非侵入性代谢成为可能， 大型动物的研究。 在大鼠、猫和恒河猴中的研究表明， 表明所有哺乳动物都具有伸长和去饱和能力， 它可以在血液供应中被监测。 这种做法使 可能的人体试验中，我们将调查酒精的影响， 滥用和戒断以及饮食对去饱和和链延长的影响。

Desaturation is a necessary step in the modification of essential fatty acids linoleic and linolenic acids to arachidonic and docosahexaenoic acid, respectively. The levels of these fatty acids are lowered by ethanol consumption in blood cells, liver and brain. The mechanisms underlying the disruption of essential fatty acid levels in these tissues is largely unknown. One possible explanation for this alcohol-induced deficiency is inhibition of elongation/desaturation of 18-carbon essential fatty acids by alcohol. A stable isotope gas chromatography/mass spectrometry (GC/MS) method has been developed that allows for the in vivo examination of these processes. This technique has improved the sensitivity of the method by several orders of magnitude and has made possible non-invasive metabolic studies in large animals. Studies in rats, cats and rhesus monkeys have indicated that all mammals have elongation and desaturation capability and that it may be monitored in the blood supply. This approach has made possible human trials in which we will investigate the effects of alcohol abuse and withdrawal as well as diet on desaturation and chain elongation.