Light-NMR spectroscopy: tools and applications

光是一种围绕并影响我们周围物质的电磁能量：无数的化学反应和生命过程都依赖于光。光很容易产生和控制，因此它可以是一个理想的触发器，将我们的宏观世界与纳米级或分子物体连接起来，调节材料特性、化学反应、分子开关、纳米器件、基因表达、靶向药物释放和许多其他物理过程。光如何调制物质，以及如何用它来控制物质，是科学中最重要的问题之一。需要新的和更强大的分析方法来揭示和密切监测光对复杂化学系统的影响，例如化学和合成生物学领域出现的光酶，以探索和优化所涉及的机制。核磁共振波谱是化学、物理和生命科学中研究物质性质和结构的最强大和最普遍的技术之一。核磁共振光谱参数对样品中存在的分子的化学和三维结构及其局部环境敏感。因此，核磁共振通常用于确认分子结构的身份、质量和纯度分析、化学反应监测以及监测构象和分子动力学的变化。该项目旨在开发将照明与核磁共振实验相结合的工具，使核磁共振的全部力量可以应用于光化学和光酶系统的研究。

Light is a form of electromagnetic energy that surrounds and affects matter around us: a myriad of chemical reactions and life processes depend on light. Light is very easy to produce and control, and therefore it can be an ideal trigger that connects our macroscopic world with nanoscale or molecular objects, to modulate material properties, chemical reactions, molecular switches, nano-devices, gene expression, targeted drug release, and many other physical processes. How light modulates matter, and how it can be used to control it, is one of the most important questions in science. New and more powerful analytical approaches are needed to reveal and closely monitor the effects of light on complex chemical systems, for example, photoenzymes emerging from chemical and synthetic biology fields, to explore and optimise the mechanisms involved. NMR spectroscopy is one of the most powerful and universal techniques in chemistry, physics and life sciences to study the properties and structure of matter. NMR spectral parameters are sensitive to chemical and 3D structure of molecules present in the samples, as well as their local environment. Therefore NMR is routinely used for confirming the identity of molecular structures, quality and purity analysis, chemical reaction monitoring, as well as monitoring the changes in conformation and molecular dynamics. The project aims at developing tools for combining illumination with NMR experiments, so that the whole power of NMR can be applied to studies of photochemical and photoenzymatic systems.